ArticleCardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
Reduced serum content and increased matrix stiffness promote the cardiac myofibroblast transition in 3D collagen matrices.
Article in Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 67 citations in OpenAlex.
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- 3D bioprinting of prefabricated artificial skin with multicomponent hydrogel for skin and hair follicle regeneration.Theranostics · 2025Article
- 3D matrix stiffness modulation unveils cardiac fibroblast phenotypic switching.Scientific reports · 2024Article
- The mechanics of the retina: Müller glia role on retinal extracellular matrix and modelling.Frontiers in medicine · 2024Review
- Materials and extracellular matrix rigidity highlighted in tissue damages and diseases: Implication for biomaterials design and therapeutic targets.Bioactive materials · 2023Review
- Culturing of Cardiac Fibroblasts in Engineered Heart Matrix Reduces Myofibroblast Differentiation but Maintains Their Response to Cyclic Stretch and Transforming Growth Factor β1.Bioengineering (Basel, Switzerland) · 2022Article
- Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles.Journal of extracellular vesicles · 2022Article
- Spatiotemporal control of myofibroblast activation in acoustically-responsive scaffolds via ultrasound-induced matrix stiffening.Acta biomaterialia · 2022Article
- Evidence for synergy between sarcomeres and fibroblasts in an in vitro model of myocardial reverse remodeling.Journal of molecular and cellular cardiology · 2021Article
- Does the Heart Want What It Wants? A Case for Self-Adapting, Mechano-Sensitive Therapies After Infarction.Frontiers in cardiovascular medicine · 2021Article
- Engineering the Cellular Microenvironment of Post-infarct Myocardium on a Chip.Frontiers in cardiovascular medicine · 2021Review
- Targeting the renin-angiotensin-aldosterone system in fibrosis.Matrix biology : journal of the International Society for Matrix Biology · 2020Review
- Potential role of transforming growth factor-beta 1/Smad signaling in secondary lymphedema after cancer surgery.Cancer science · 2020Article
- Spatiotemporal delivery of basic fibroblast growth factor to directly and simultaneously attenuate cardiac fibrosis and promote cardiac tissue vascularization following myocardial infarction.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Potential strain-dependent mechanisms defining matrix alignment in healing tendons.Biomechanics and modeling in mechanobiology · 2018Article
- An Injectable Oxygen Release System to Augment Cell Survival and Promote Cardiac Repair Following Myocardial Infarction.Scientific reports · 2018Article
- Stem Cell Differentiation is Regulated by Extracellular Matrix Mechanics.Physiology (Bethesda, Md.) · 2018Review
- Investigation of cardiac fibroblasts using myocardial slices.Cardiovascular research · 2018Article
- Mechanically tuned 3 dimensional hydrogels support human mammary fibroblast growth and viability.BMC cell biology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
introductionThe fibroblast-myofibroblast transition is an important event in the development of cardiac fibrosis and scar formation initiated after myocardial ischemia. The goals of the present study were to better understand the contribution of environmental factors to this transition and determine whether myofibroblasts provide equally important feedback to the surrounding environment.
methodsThe influence of matrix stiffness and serum concentration on the myofibroblast transition was assessed by measuring message levels of a panel of cardiac fibroblast phenotype markers using quantitative reverse transcriptase polymerase chain reaction. Cell-mediated gel compaction measured the influence of environmental factors on cardiac fibroblast contractility. Immunohistochemistry characterized alpha-smooth muscle actin expression and cell morphology, while static and dynamic compression testing evaluated the effect of the cell response on the mechanical properties of the cell-seeded collagen hydrogels.
resultsBoth reduced serum content and increased matrix stiffness contributed to the myofibroblast transition, as indicated by contractile compaction of the gels, increased message levels of col3α1 and alpha-smooth muscle actin, and a less stellate morphology. However, the effects of serum and matrix stiffness were not additive. Mechanical testing indicated that reduced serum content increased the initial elastic modulus of cell-seeded gels and that gels lost their viscous character with time.
conclusionsThe results suggest that reduced serum and increased matrix stiffness promote the myofibroblast phenotype in the myocardium. This transition both enhances and is promoted by matrix stiffness, indicating the presence of positive feedback that may contribute to the pathogenesis of cardiac fibrosis.
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