ArticleMolecular microbiology2011
Oligonucleotides stimulate genomic alterations of Legionella pneumophila.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- SHARK: A Specialized Host for Assembling R6K Plasmids.ACS synthetic biology · 2026Article
- Recombineering in Non-Model Bacteria.Current protocols · 2022Article
- Host Adaptation in Legionellales Is 1.9 Ga, Coincident with Eukaryogenesis.Molecular biology and evolution · 2022Article
- Recombineering and MAGE.Nature reviews. Methods primers · 2021Article
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- Stenotrophomonas maltophilia Encodes a VirB/VirD4 Type IV Secretion System That Modulates Apoptosis in Human Cells and Promotes Competition against Heterologous Bacteria, Including Pseudomonas aeruginosa.Infection and immunity · 2019Article
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli.Applied and environmental microbiology · 2015Article
- Horizontal transfer of short and degraded DNA has evolutionary implications for microbes and eukaryotic sexual reproduction.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
- A Brief Review: The Z-curve Theory and its Application in Genome Analysis.Current genomics · 2014Article
- Bacterial natural transformation by highly fragmented and damaged DNA.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits.Infection and immunity · 2013Article
- Substrate and target sequence length influence RecTE(Psy) recombineering efficiency in Pseudomonas syringae.PloS one · 2012Article
- Efficient generation of unmarked deletions in Legionella pneumophila.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Genetic variation generates diversity in all kingdoms of life. The corresponding mechanisms can also be harnessed for laboratory studies of fundamental cellular processes. Here we report that oligonucleotides (oligos) generate mutations on the Legionella pneumophila chromosome by a mechanism that requires homologous DNA, but not RecA, RadA or any known phage recombinase. Instead we propose that DNA replication contributes, as oligo-induced mutagenesis required ≥ 21 nucleotides of homology, was strand-dependent, and was most efficient in exponential phase. Mutagenesis did not require canonical 5' phosphate or 3' hydroxyl groups, but the primosomal protein PriA and DNA Pol I contributed. After electroporation, oligos stimulated excision of 2.1 kb of chromosomal DNA or insertion of 18 bp, and non-homologous flanking sequences were also processed. We exploited this endogenous activity to generate chromosomal deletions and to insert an epitope into a chromosomal coding sequence. Compared with Escherichia coli, L. pneumophila encodes fewer canonical single-stranded exonucleases, and the frequency of mutagenesis increased substantially when either its RecJ and ExoVII nucleases were inactivated or the oligos modified by nuclease-resistant bases. In addition to genetic engineering, oligo-induced mutagenesis may have evolutionary implications as a mechanism to incorporate divergent DNA sequences with only short regions of homology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.