ArticleThe International journal of developmental biology2010
Porcine induced pluripotent stem cells analogous to naïve and primed embryonic stem cells of the mouse.
Article in The International journal of developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 108 citations in OpenAlex.
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions.Stem cell reviews and reports · 2022Article
- iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.Biomedicines · 2021Review
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Exogenous LIN28 Is Required for the Maintenance of Self-Renewal and Pluripotency in Presumptive Porcine-Induced Pluripotent Stem Cells.Frontiers in cell and developmental biology · 2021Article
- The use of induced pluripotent stem cells in domestic animals: a narrative review.BMC veterinary research · 2020Review
- IRF-1 expressed in the inner cell mass of the porcine early blastocyst enhances the pluripotency of induced pluripotent stem cells.Stem cell research & therapy · 2020Article
- Induced pluripotent stem cells from farm animals.Journal of animal science · 2020Review
- Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells.Stem cells and development · 2019Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Establishment of porcine and human expanded potential stem cells.Nature cell biology · 2019Article
- Preserving self-renewal of porcine pluripotent stem cells in serum-free 3i culture condition and independent of LIF and b-FGF cytokines.Cell death discovery · 2018Article
- Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish.Journal of animal science and biotechnology · 2018Review
- Generation of transgene-free porcine intermediate type induced pluripotent stem cells.Cell cycle (Georgetown, Tex.) · 2018Article
- Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering.Biomaterials · 2017Article
- Genome editing and genetic engineering in livestock for advancing agricultural and biomedical applications.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Identification of SSEA-1 expressing enhanced reprogramming (SEER) cells in porcine embryonic fibroblasts.Cell cycle (Georgetown, Tex.) · 2017Article
- EpCAM Intracellular Domain Promotes Porcine Cell Reprogramming by Upregulation of Pluripotent Gene Expression via Beta-catenin Signaling.Scientific reports · 2017Article
- Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells.Molecular reproduction and development · 2017Article
- Epigenetic Modifiers Facilitate Induction and Pluripotency of Porcine iPSCs.Stem cell reports · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Authentic or naïve embryonic stem cells (ESC) have probably never been derived from the inner cell mass (ICM) of pig blastocysts, despite over 25 years of effort. Recently, several groups, including ours, have reported induced pluripotent stem cells (iPSC) from swine by reprogramming somatic cells with a combination of four factors, OCT4 (POU5F1)/SOX2/KLF4/c-MYC delivered by retroviral transduction. The porcine (p) iPSC resembled human (h) ESC and the mouse "Epiblast stem cells" (EpiSC) in their colony morphology and expression of pluripotent genes, and are likely dependent on FGF2/ACTIVIN/NODAL signaling, therefore representing a primed ESC state. These cells are likely to advance swine as a model in biomedical research, since grafts could potentially be matched to the animal that donated the cells for re-programming. The objective of the present work has been to develop naïve piPSC. Employing a combination of seven reprogramming factors assembled on episomal vectors, we successfully reprogrammed porcine embryonic fibroblasts on a modified LIF-medium supplemented with two kinase inhibitors; CHIR99021, which inhibits GSK-3beta, and PD0325901, a MEK inhibitor. The derived piPSC bear a striking resemblance to naïve mESC in colony morphology, are dependent on LIF to maintain an undifferentiated phenotype, and express markers consistent with pluripotency. They exhibit high telomerase activity, a short cell cycle interval, and a normal karyotype, and are able to generate teratomas. Currently, the competence of these lines for contributing to germ-line chimeras is being tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.