ArticlePloS one2011
IS-linked movement of a restriction-modification system.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 17 citations in OpenAlex.
- Epigenetic regulation and antimicrobial resistance: functional roles of DNA methylation.FEMS microbiology reviews · 2026Review
- In silico approaches for discovering microbial antiviral defense systems.Briefings in bioinformatics · 2025Review
- Article
- Comparative Genomic Analysis ofInternational journal of genomics · 2019Article
- Evolution of Immune Systems From Viruses and Transposable Elements.Frontiers in microbiology · 2019Review
- Prokaryotic horizontal gene transfer within the human holobiont: ecological-evolutionary inferences, implications and possibilities.Microbiome · 2018Review
- Kinetic Basis of the Bifunctionality of SsoII DNA Methyltransferase.Molecules (Basel, Switzerland) · 2018Article
- Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Restriction-Modification Systems as Mobile Genetic Elements in the Evolution of an Intracellular Symbiont.Molecular biology and evolution · 2016Article
- Enhanced production of recombinant proteins with Corynebacterium glutamicum by deletion of insertion sequences (IS elements).Microbial cell factories · 2015Article
- Phylogenomics and sequence-structure-function relationships in the GmrSD family of Type IV restriction enzymes.BMC bioinformatics · 2015Article
- The interplay of restriction-modification systems with mobile genetic elements and their prokaryotic hosts.Nucleic acids research · 2014Article
- Bacteriophage orphan DNA methyltransferases: insights from their bacterial origin, function, and occurrence.Applied and environmental microbiology · 2013Review
- Comparative genomics of defense systems in archaea and bacteria.Nucleic acids research · 2013Review
- Defense islands in bacterial and archaeal genomes and prediction of novel defense systems.Journal of bacteriology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature-sensitive plasmid carrying a PaeR7I restriction-modification system in Escherichia coli cells, the plasmid was found integrated into the chromosome of the surviving cells. Sequence analysis revealed that, in the majority of products, the restriction-modification system was linked to chromosomal insertion sequences (ISs). Three types of products were: (I) apparent co-integration of the plasmid and the chromosome at a chromosomal IS1 or IS5 copy (24/28 analyzed); (II) de novo insertion of IS1 with the entire plasmid except for a 1-3 bp terminal deletion (2/28); and (III) reciprocal crossing-over between the plasmid and the chromosome involving 1-3 bp of sequence identity (2/28). An R-negative mutation apparently decreased the efficiency of successful integration by two orders of magnitude. Reconstruction experiments demonstrated that the restriction-dependence was mainly due to selection against cells without proper integration: their growth was inhibited by the restriction enzyme action. These results demonstrate collaboration of a mobile element and a restriction-modification system for successful joint migration. This collaboration may have promoted the spread and, therefore, the long-term persistence of these complexes and restriction-modification systems in a wide range of prokaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.