ArticlePloS one2011
siRNA-mediated gene targeting in Aedes aegypti embryos reveals that frazzled regulates vector mosquito CNS development.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- DsiRNA-mediated silencing of Ceratitis capitata transformer or transformer-2 leads to masculinization of XX embryos and systemic gene silencing in ovaries.Insect science · 2026Article
- Evolutionary conservation of midline axon guidance activity between Drosophila and Tribolium Frazzled.Developmental biology · 2026Article
- RNA Interference Reveals the Impacts ofInsects · 2024Article
- Article
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- Coupled small molecules target RNA interference and JAK/STAT signaling to reduce Zika virus infection in Aedes aegypti.PLoS pathogens · 2022Article
- Advances in oral RNAi for disease vector mosquito research and control.Current opinion in insect science · 2020Review
- Midline axon guidance in the Drosophila embryonic central nervous system.Seminars in cell & developmental biology · 2019Review
- Preparation and Use of a Yeast shRNA Delivery System for Gene Silencing in Mosquito Larvae.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Yeast interfering RNA larvicides targeting neural genes induce high rates of Anopheles larval mortality.Malaria journal · 2017Article
- Review
- Redeployment of a conserved gene regulatory network during Aedes aegypti development.Developmental biology · 2016Article
- High-throughput cis-regulatory element discovery in the vector mosquito Aedes aegypti.BMC genomics · 2016Article
- Chitosan/interfering RNA nanoparticle mediated gene silencing in disease vector mosquito larvae.Journal of visualized experiments : JoVE · 2015Article
- Chitosan/siRNA nanoparticle targeting demonstrates a requirement for single-minded during larval and pupal olfactory system development of the vector mosquito Aedes aegypti.BMC developmental biology · 2014Article
- Examination of the genetic basis for sexual dimorphism in the Aedes aegypti (dengue vector mosquito) pupal brain.Biology of sex differences · 2014Article
- Requirement for commissureless2 function during dipteran insect nerve cord development.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Stage-specific transcription during development of Aedes aegypti.BMC developmental biology · 2013Article
- Article
- Disruption of Aedes aegypti olfactory system development through chitosan/siRNA nanoparticle targeting of semaphorin-1a.PLoS neglected tropical diseases · 2013Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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Abstract
Although mosquito genome projects uncovered orthologues of many known developmental regulatory genes, extremely little is known about the development of vector mosquitoes. Here, we investigate the role of the Netrin receptor frazzled (fra) during embryonic nerve cord development of two vector mosquito species. Fra expression is detected in neurons just prior to and during axonogenesis in the embryonic ventral nerve cord of Aedes aegypti (dengue vector) and Anopheles gambiae (malaria vector). Analysis of fra function was investigated through siRNA-mediated knockdown in Ae. aegypti embryos. Confirmation of fra knockdown, which was maintained throughout embryogenesis, indicated that microinjection of siRNA is an effective method for studying gene function in Ae. aegypti embryos. Loss of fra during Ae. aegypti development results in thin and missing commissural axons. These defects are qualitatively similar to those observed in Dr. melanogaster fra null mutants. However, the Aa. aegypti knockdown phenotype is stronger and bears resemblance to the Drosophila commissureless mutant phenotype. The results of this investigation, the first targeted knockdown of a gene during vector mosquito embryogenesis, suggest that although Fra plays a critical role during development of the Ae. aegypti ventral nerve cord, mechanisms regulating embryonic commissural axon guidance have evolved in distantly related insects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.