ArticlePLoS pathogens2011
Charge-surrounded pockets and electrostatic interactions with small ions modulate the activity of retroviral fusion proteins.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Characterization and application of recombinant Bovine Leukemia Virus Env protein.Scientific reports · 2024Article
- A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.Biomolecules · 2023Review
- Unique Structure and Distinctive Properties of the Ancient and Ubiquitous Gamma-Type Envelope Glycoprotein.Viruses · 2023Review
- Kinetics of Bovine leukemia virus aspartic protease reveals its dimerization and conformational change.PloS one · 2022Article
- X-ray Structures of the Post-fusion 6-Helix Bundle of the Human Syncytins and their Functional Implications.Journal of molecular biology · 2019Article
- In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51.PloS one · 2018Article
- Crystal structures of MBP fusion proteins.Protein science : a publication of the Protein Society · 2016Review
- Sequence variation of koala retrovirus transmembrane protein p15E among koalas from different geographic regions.Virology · 2015Article
- Ebola hemorrhagic fever: Properties of the pathogen and development of vaccines and chemotherapeutic agents.Molecular biology · 2015Review
- Structural characterization of the glycoprotein GP2 core domain from the CAS virus, a novel arenavirus-like species.Journal of molecular biology · 2014Article
- Crystal structures of beta- and gammaretrovirus fusion proteins reveal a role for electrostatic stapling in viral entry.Journal of virology · 2014Article
- Structural characterization of a fusion glycoprotein from a retrovirus that undergoes a hybrid 2-step entry mechanism.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- A detailed molecular analysis of complete bovine leukemia virus genomes isolated from B-cell lymphosarcomas.Veterinary research · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Refolding of viral class-1 membrane fusion proteins from a native state to a trimer-of-hairpins structure promotes entry of viruses into cells. Here we present the structure of the bovine leukaemia virus transmembrane glycoprotein (TM) and identify a group of asparagine residues at the membrane-distal end of the trimer-of-hairpins that is strikingly conserved among divergent viruses. These asparagines are not essential for surface display of pre-fusogenic envelope. Instead, substitution of these residues dramatically disrupts membrane fusion. Our data indicate that, through electrostatic interactions with a chloride ion, the asparagine residues promote assembly and profoundly stabilize the fusion-active structures that are required for viral envelope-mediated membrane fusion. Moreover, the BLV TM structure also reveals a charge-surrounded hydrophobic pocket on the central coiled coil and interactions with basic residues that cluster around this pocket are critical to membrane fusion and form a target for peptide inhibitors of envelope function. Charge-surrounded pockets and electrostatic interactions with small ions are common among class-1 fusion proteins, suggesting that small molecules that specifically target such motifs should prevent assembly of the trimer-of-hairpins and be of value as therapeutic inhibitors of viral entry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.