ArticlePLoS pathogens2011
In situ photodegradation of incorporated polyanion does not alter prion infectivity.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Cofactor molecules: Essential partners for infectious prions.Progress in molecular biology and translational science · 2020Review
- In vitro Modeling of Prion Strain Tropism.Viruses · 2019Review
- Mutations Alter RNA-Mediated Conversion of Human Prions.ACS omega · 2018Article
- Molecular dynamics simulations of early steps in RNA-mediated conversion of prions.Protein science : a publication of the Protein Society · 2017Article
- Prion Strain Diversity.Cold Spring Harbor perspectives in medicine · 2016Review
- A Structural and Functional Comparison Between Infectious and Non-Infectious Autocatalytic Recombinant PrP Conformers.PLoS pathogens · 2015Article
- Prion nucleation site unmasked by transient interaction with phospholipid cofactor.Biochemistry · 2014Article
- Changes in prion replication environment cause prion strain mutation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Role of lipid in forming an infectious prion?Acta biochimica et biophysica Sinica · 2013Review
- Cofactor molecules maintain infectious conformation and restrict strain properties in purified prions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- High-resolution structure of infectious prion protein: the final frontier.Nature structural & molecular biology · 2012Review
- Genetic informational RNA is not required for recombinant prion infectivity.Journal of virology · 2012Article
- The role of cofactors in prion propagation and infectivity.PLoS pathogens · 2012Review
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Single-stranded polyanions ≥40 bases in length facilitate the formation of hamster scrapie prions in vitro, and polyanions co-localize with PrP(Sc) aggregates in vivo. To test the hypothesis that intact polyanionic molecules might serve as a structural backbone essential for maintaining the infectious conformation(s) of PrP(Sc), we produced synthetic prions using a photocleavable, 100-base oligonucleotide (PC-oligo). In serial Protein Misfolding Cyclic Amplification (sPMCA) reactions using purified PrP(C) substrate, PC-oligo was incorporated into physical complexes with PrP(Sc) molecules that were resistant to benzonase digestion. Exposure of these nuclease-resistant prion complexes to long wave ultraviolet light (315 nm) induced degradation of PC-oligo into 5 base fragments. Light-induced photolysis of incorporated PC-oligo did not alter the infectivity of in vitro-generated prions, as determined by bioassay in hamsters and brain homogenate sPMCA assays. Neuropathological analysis also revealed no significant differences in the neurotropism of prions containing intact versus degraded PC-oligo. These results show that polyanions >5 bases in length are not required for maintaining the infectious properties of in vitro-generated scrapie prions, and indicate that such properties are maintained either by short polyanion remnants, other co-purified cofactors, or by PrP(Sc) molecules alone.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.