ArticleStandards in genomic sciences2010
Two genome sequences of the same bacterial strain, Gluconacetobacter diazotrophicus PAl 5, suggest a new standard in genome sequence submission.
Article in Standards in genomic sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- Overcoming urea and nitrate inhibition of biological nitrogen fixation in Azotobacter vinelandii versus natural tolerance in Gluconacetobacter diazotrophicus.World journal of microbiology & biotechnology · 2026Article
- A deoxyviolacein-based transposon insertion vector for pigmented tracer studies.MicrobiologyOpen · 2024Article
- Enhanced extracellular ammonium release in the plant endophyteMicrobiology spectrum · 2024Article
- Gluconacetobacter diazotrophicus Gene Fitness during Diazotrophic Growth.Applied and environmental microbiology · 2022Article
- Dissection and Reconstitution Provide Insights into Electron Transport in the Membrane-Bound Aldehyde Dehydrogenase Complex of Gluconacetobacter diazotrophicus.Journal of bacteriology · 2022Article
- Elimination of aromatic fusel alcohols as by-products ofMetabolic engineering communications · 2021Article
- Whole genome analysis of3 Biotech · 2021Article
- Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244.Applied microbiology and biotechnology · 2021Article
- Phylogenomic Framework for Taxonomic Delineation ofIndian journal of microbiology · 2021Article
- Molecular Cloning and Exploration of the Biochemical and Functional Analysis of Recombinant Glucose-6-Phosphate Dehydrogenase fromInternational journal of molecular sciences · 2019Article
- Genomic analysis of bacteria in the Acute Oak Decline pathobiome.Microbial genomics · 2019Article
- Conformationally Gated Electron Transfer in Nitrogenase. Isolation, Purification, and Characterization of Nitrogenase From Gluconacetobacter diazotrophicus.Methods in enzymology · 2018Article
- Complete genome and gene expression analyses of Asaia bogorensis reveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- CVTree3 Web Server for Whole-genome-based and Alignment-free Prokaryotic Phylogeny and Taxonomy.Genomics, proteomics & bioinformatics · 2015Article
- De novo assembly of Dekkera bruxellensis: a multi technology approach using short and long-read sequencing and optical mapping.GigaScience · 2015Article
- Comparative Genomics Reveal That Host-Innate Immune Responses Influence the Clinical Prevalence of Legionella pneumophila Serogroups.PloS one · 2013Article
- Enhanced de novo assembly of high throughput pyrosequencing data using whole genome mapping.PloS one · 2013Article
- Rapid genomic-scale analysis of Escherichia coli O104:H4 by using high-resolution alternative methods to next-generation sequencing.Applied and environmental microbiology · 2012Article
- Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.Applied and environmental microbiology · 2011Article
- Rapid whole genome optical mapping of Plasmodium falciparum.Malaria journal · 2011Article
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4 authors.
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Abstract
Gluconacetobacter diazotrophicus PAl 5 is of agricultural significance due to its ability to provide fixed nitrogen to plants. Consequently, its genome sequence has been eagerly anticipated to enhance understanding of endophytic nitrogen fixation. Two groups have sequenced the PAl 5 genome from the same source (ATCC 49037), though the resulting sequences contain a surprisingly high number of differences. Therefore, an optical map of PAl 5 was constructed in order to determine which genome assembly more closely resembles the chromosomal DNA by aligning each sequence against a physical map of the genome. While one sequence aligned very well, over 98% of the second sequence contained numerous rearrangements. The many differences observed between these two genome sequences could be owing to either assembly errors or rapid evolutionary divergence. The extent of the differences derived from sequence assembly errors could be assessed if the raw sequencing reads were provided by both genome centers at the time of genome sequence submission. Hence, a new genome sequence standard is proposed whereby the investigator supplies the raw reads along with the closed sequence so that the community can make more accurate judgments on whether differences observed in a single stain may be of biological origin or are simply caused by differences in genome assembly procedures.
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