ArticlePloS one2011
DNA topoisomerase II modulates insulator function in Drosophila.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila.Cell death & disease · 2021Article
- Multi-Scale Organization of theGenes · 2021Review
- Adenine Methylation inG3 (Bethesda, Md.) · 2019Article
- Article
- Sex Differences in Drosophila melanogaster Heterochromatin Are Regulated by Non-Sex Specific Factors.PloS one · 2015Article
- Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function.Cellular and molecular life sciences : CMLS · 2014Review
- Topoisomerase II is required for the proper separation of heterochromatic regions during Drosophila melanogaster female meiosis.PLoS genetics · 2014Article
- The analysis of mutant alleles of different strength reveals multiple functions of topoisomerase 2 in regulation of Drosophila chromosome structure.PLoS genetics · 2014Article
- dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes.Nucleic acids research · 2014Article
- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- Chromatin insulator bodies are nuclear structures that form in response to osmotic stress and cell death.The Journal of cell biology · 2013Article
- Genome-wide localization of exosome components to active promoters and chromatin insulators in Drosophila.Nucleic acids research · 2013Article
- Identification and characterization of proteins involved in nuclear organization using Drosophila GFP protein trap lines.PloS one · 2013Article
- Restoration of topoisomerase 2 function by complementation of defective monomers in Drosophila.Genetics · 2012Article
- Identification of genes that promote or antagonize somatic homolog pairing using a high-throughput FISH-based screen.PLoS genetics · 2012Article
- Tissue-specific regulation of chromatin insulator function.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Insulators are DNA sequences thought to be important for the establishment and maintenance of cell-type specific nuclear architecture. In Drosophila there are several classes of insulators that appear to have unique roles in gene expression. The mechanisms involved in determining and regulating the specific roles of these insulator classes are not understood. Here we report that DNA Topoisomerase II modulates the activity of the Su(Hw) insulator. Downregulation of Topo II by RNAi or mutations in the Top2 gene result in disruption of Su(Hw) insulator function. This effect is mediated by the Mod(mdg4)2.2 protein, which is a unique component of the Su(Hw) insulator complex. Co-immunoprecipitation and yeast two-hybrid experiments show that Topo II and Mod(mdg4)2.2 proteins directly interact. In addition, mutations in Top2 cause a slight decrease of Mod(mdg4)2.2 transcript but have a dramatic effect on Mod(mdg4)2.2 protein levels. In the presence of proteasome inhibitors, normal levels of Mod(mdg4)2.2 protein and its binding to polytene chromosomes are restored. Thus, Topo II is required to prevent Mod(mdg4)2.2 degradation and, consequently, to stabilize Su(Hw) insulator-mediated chromatin organization.
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