ArticleJournal of visualized experiments : JoVE2011
Dissection of human vitreous body elements for proteomic analysis.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- In Vivo Functional and Structural Retinal Preservation by Combined Administration of Citicoline and Coenzyme Q10 in a Murine Model of Ocular Hypertension.International journal of molecular sciences · 2026Article
- Advances of Intraocular Fluid for the Application and Treatment of Uveitis.Clinical ophthalmology (Auckland, N.Z.) · 2026Review
- An Exploratory Study Provides Insights into MMP9 and Aβ Levels in the Vitreous and Blood across Different Ages and in a Subset of AMD Patients.International journal of molecular sciences · 2022Article
- Investigation of Cas9 antibodies in the human eye.Nature communications · 2022Article
- Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging.Biomedicines · 2021Article
- Proteomic profile of vitreous in patients with tubercular uveitis.Tuberculosis (Edinburgh, Scotland) · 2021Article
- Protein Biomarkers in Uveitis.Frontiers in immunology · 2020Review
- Fibrin Glue and Internal Limiting Membrane Abrasion for Optic Disc Pit Maculopathy.Ophthalmic surgery, lasers & imaging retina · 2018Article
- Extracellular superoxide dismutase (SOD3) regulates oxidative stress at the vitreoretinal interface.Free radical biology & medicine · 2018Article
- Dissection of Human Retina and RPE-Choroid for Proteomic Analysis.Journal of visualized experiments : JoVE · 2017Article
- Isotope-coded protein label based quantitative proteomic analysis reveals significant up-regulation of apolipoprotein A1 and ovotransferrin in the myopic chick vitreous.Scientific reports · 2017Article
- Identification of Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Novel Neuroprotective Factor for Retinal Ganglion Cells.Frontiers in molecular neuroscience · 2017Article
- Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid.Proteomics · 2016Article
- Differential Expression of Vitreous Proteins in Young and Mature New Zealand White Rabbits.PloS one · 2016Article
- Spatial Variations in Vitreous Oxygen Consumption.PloS one · 2016Article
- Whole vitreous humor dissection for vitreodynamic analysis.Journal of visualized experiments : JoVE · 2015Article
- The proteome of human retina.Proteomics · 2015Article
- Article
- Translational vitreous proteomics.Proteomics. Clinical applications · 2014Article
- A metabolomic approach to animal vitreous humor topographical composition: a pilot study.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The vitreous is an optically clear, collagenous extracellular matrix that fills the inside of the eye and overlies the retina. (1,2) Abnormal interactions between vitreous substructures and the retina underlie several vitreoretinal diseases, including retinal tear and detachment, macular pucker, macular hole, age-related macular degeneration, vitreomacular traction, proliferative vitreoretinopathy, proliferative diabetic retinopathy, and inherited vitreoretinopathies. (1,2) The molecular composition of the vitreous substructures is not known. Since the vitreous body is transparent with limited surgical access, it has been difficult to study its substructures at the molecular level. We developed a method to separate and preserve these tissues for proteomic and biochemical analysis. The dissection technique in this experimental video shows how to isolate vitreous base, anterior hyaloid, vitreous core, and vitreous cortex from postmortem human eyes. One-dimensional SDS-PAGE analyses of each vitreous component showed that our dissection technique resulted in four unique protein profiles corresponding to each substructure of the human vitreous body. Identification of differentially compartmentalized proteins will reveal candidate molecules underlying various vitreoretinal diseases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.