ArticlePhysical biology2011
Promotion of experimental thrombus formation by the procoagulant activity of breast cancer cells.
Article in Physical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Lipid Transporters Beam Signals from Cell Membranes.Membranes · 2021Review
- Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo.Scientific reports · 2020Article
- A Direct Podocalyxin-Dynamin-2 Interaction Regulates Cytoskeletal Dynamics to Promote Migration and Metastasis in Pancreatic Cancer Cells.Cancer research · 2019Article
- The role of coagulation and platelets in colon cancer-associated thrombosis.American journal of physiology. Cell physiology · 2019Article
- The thrombotic potential of circulating tumor microemboli: computational modeling of circulating tumor cell-induced coagulation.American journal of physiology. Cell physiology · 2015Article
- The BCR-ABL inhibitor ponatinib inhibits platelet immunoreceptor tyrosine-based activation motif (ITAM) signaling, platelet activation and aggregate formation under shear.Thrombosis research · 2015Article
- Physical biology in cancer. 2. The physical biology of circulating tumor cells.American journal of physiology. Cell physiology · 2014Review
- Interrelationships of circulating tumor cells with metastasis and thrombosis: role of microRNAs.Current pharmaceutical design · 2014Review
- c-Jun N-Terminal Kinases Mediate a Wide Range of Targets in the Metastatic Cascade.Genes & cancer · 2013Review
- Physiological levels of blood coagulation factors IX and X control coagulation kinetics in an in vitro model of circulating tissue factor.Physical biology · 2013Article
- Inhibition of metastatic tumor formation in vivo by a bacteriophage display-derived galectin-3 targeting peptide.Clinical & experimental metastasis · 2013Article
- Thrombin generation assay and transmission electron microscopy: a useful combination to study tissue factor-bearing microvesicles.Journal of extracellular vesicles · 2013Article
- Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors.Physical biology · 2012Article
- Do circulating tumor cells play a role in coagulation and thrombosis?Frontiers in oncology · 2012Article
- Development of coagulation factor probes for the identification of procoagulant circulating tumor cells.Frontiers in oncology · 2012Article
- Modeling and simulation of procoagulant circulating tumor cells in flow.Frontiers in oncology · 2012Article
- The role of carrier number on the procoagulant activity of tissue factor in blood and plasma.Physical biology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The routine observation of tumor emboli in the peripheral blood of patients with carcinomas raises questions about the clinical relevance of these circulating tumor cells. Thrombosis is a common clinical manifestation of cancer, and circulating tumor cells may play a pathogenetic role in this process. The presence of coagulation-associated molecules on cancer cells has been described, but the mechanisms by which circulating tumor cells augment or alter coagulation remains unclear. In this study we utilized suspensions of a metastatic adenocarcinoma cell line, MDA-MB-231, and a non-metastatic breast epithelial cell line, MCF-10A, as models of circulating tumor cells to determine the thrombogenic activity of these blood-foreign cells. In human plasma, both metastatic MDA-MB-231 cells and non-metastatic MCF-10A cells significantly enhanced clotting kinetics. The effect of MDA-MB-231 and MCF-10A cells on clotting times was cell number-dependent and inhibited by a neutralizing antibody to tissue factor (TF) as well as inhibitors of activated factor X and thrombin. Using fluorescence microscopy, we found that both MDA-MB-231 and MCF-10A cells supported the binding of fluorescently labeled thrombin. Furthermore, in a model of thrombus formation under pressure-driven flow, MDA-MB-231 and MCF-10A cells significantly decreased the time to occlusion. Our findings indicate that the presence of breast epithelial cells in blood can stimulate coagulation in a TF-dependent manner, suggesting that tumor cells that enter the circulation may promote the formation of occlusive thrombi under shear flow conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.