ArticleProceedings of the National Academy of Sciences of the United States of America2011
Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
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- Antimicrobial dual-crosslinked hydrogel synergizes bioengineered extracellular vesicles for enhanced diabetic wound healing.Materials today. Bio · 2025Article
- From Crypts to Cancer: A Holistic Perspective on Colorectal Carcinogenesis and Therapeutic Strategies.International journal of molecular sciences · 2024Review
- Cardiac differentiation is modulated by anti-apoptotic signals in murine embryonic stem cells.World journal of stem cells · 2024Article
- NELFA and BCL2 induce the 2C-like state in mouse embryonic stem cells in a chemically defined medium.Cell proliferation · 2024Article
- Mycelium-based biomaterials as smart devices for skin wound healing.Frontiers in bioengineering and biotechnology · 2023Article
- Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos.Cell reports · 2022Article
- Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction.Scientific reports · 2022Article
- AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).International journal of molecular sciences · 2021Article
- Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior.Genes & development · 2021Article
- BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2.Cell proliferation · 2021Article
- Acquired genetic changes in human pluripotent stem cells: origins and consequences.Nature reviews. Molecular cell biology · 2020Review
- Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells.American journal of translational research · 2020Article
- Highly efficient genome editing via CRISPR-Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression.Nucleic acids research · 2018Article
- Matched Developmental Timing of Donor Cells with the Host Is Crucial for Chimera Formation.Stem cell reports · 2018Article
- Molecular and epigenetic regulatory mechanisms of normal stem cell radiosensitivity.Cell death discovery · 2018Review
- Article
- Association of TGFβ signaling with the maintenance of a quiescent stem cell niche in human oral mucosa.Histochemistry and cell biology · 2016Article
- AKT/GSK3β signaling pathway is critically involved in human pluripotent stem cell survival.Scientific reports · 2016Article
- Protein kinase A inhibitor, H89, significantly enhances survival rate of dissociated human embryonic stem cells following cryopreservation.Cell proliferation · 2016Article
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6 authors at 2 institutions in 2 countries.
Funding
Abstract
The promise of pluripotent stem cells as a research and therapeutic tool is partly undermined by the technical challenges of generating and maintaining these cells in culture. Human embryonic stem cells (hESCs) are exquisitely sensitive to culture conditions, and require constant signaling by growth factors and cell-cell and cell-matrix interactions to prevent apoptosis, senescence, and differentiation. Previous work from our laboratory demonstrated that overexpression of the prosurvival gene BCL2 in mouse embryonic stem cells overrode the requirement of serum factors and feeder cells to maintain mESCs in culture. To determine whether this prosurvival gene could similarly protect hESCs, we generated hESC lines that constitutively or inducibly express BCL2. We find that BCL2 overexpression significantly decreases dissociation-induced apoptosis, resulting in enhanced colony formation from sorted single cells, and enhanced embryoid body formation. In addition, BCL2-hESCs exhibit normal growth in the absence of serum, but require basic fibroblast growth factor to remain undifferentiated. Furthermore, they maintain their pluripotency markers, form teratomas in vivo, and differentiate into all three germ layers. Our data suggest that the BCL2 signaling pathway plays an important role in inhibiting hESC apoptosis, such that its overexpression in hESCs offers both a survival benefit in conditions of stress by resisting apoptosis and obviates the requirement for serum or a feeder layer for maintenance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.