ArticleProceedings of the National Academy of Sciences of the United States of America2011
Geminin promotes neural fate acquisition of embryonic stem cells by maintaining chromatin in an accessible and hyperacetylated state.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
- Intrinsic neural stem cell properties define brain hypersensitivity to genotoxic stress.Stem cell reports · 2022Article
- A pathogenic DYT-THAP1 dystonia mutation causes hypomyelination and loss of YY1 binding.Human molecular genetics · 2022Article
- Gene, cell, and organ multiplication drives inner ear evolution.Developmental biology · 2017Review
- Geminin deletion in pre-meiotic DNA replication stage causes spermatogenesis defect and infertility.The Journal of reproduction and development · 2017Article
- Geminin deficiency enhances survival in a murine medulloblastoma model by inducing apoptosis of preneoplastic granule neuron precursors.Genes & cancer · 2017Article
- The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.Developmental cell · 2017Article
- Foxd4 is essential for establishing neural cell fate and for neuronal differentiation.Genesis (New York, N.Y. : 2000) · 2017Article
- Geminin facilitates FoxO3 deacetylation to promote breast cancer cell metastasis.The Journal of clinical investigation · 2017Article
- Dual roles of Akirin2 protein duringThe Journal of biological chemistry · 2017Article
- Geminin Is Essential for Pluripotent Cell Viability During Teratoma Formation, but Not for Differentiated Cell Viability During Teratoma Expansion.Stem cells and development · 2017Article
- Review
- Gene regulatory networks in neural cell fate acquisition from genome-wide chromatin association of Geminin and Zic1.Scientific reports · 2016Article
- Gene regulation and genetics in neurochemistry, past to future.Journal of neurochemistry · 2016Review
- GemC1 controls multiciliogenesis in the airway epithelium.EMBO reports · 2016Article
- Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin.PloS one · 2016Article
- Neural transcription factors: from embryos to neural stem cells.Molecules and cells · 2014Review
- Geminin loss causes neural tube defects through disrupted progenitor specification and neuronal differentiation.Developmental biology · 2014Article
- Transcription of the Geminin gene is regulated by a negative-feedback loop.Molecular biology of the cell · 2014Article
- A complex regulatory network coordinating cell cycles during C. elegans development is revealed by a genome-wide RNAi screen.G3 (Bethesda, Md.) · 2014Article
- The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Formation of the complex vertebrate nervous system begins when pluripotent cells of the early embryo are directed to acquire a neural fate. Although cell intrinsic controls play an important role in this process, the molecular nature of this regulation is not well defined. Here we assessed the role for Geminin, a nuclear protein expressed in embryonic cells, during neural fate acquisition from mouse embryonic stem (ES) cells. Whereas Geminin knockdown does not affect the ability of ES cells to maintain or exit pluripotency, we found that it significantly impairs their ability to acquire a neural fate. Conversely, Geminin overexpression promotes neural gene expression, even in the presence of growth factor signaling that antagonizes neural transcriptional responses. These data demonstrate that Geminin's activity contributes to mammalian neural cell fate acquisition. We investigated the mechanistic basis of this phenomenon and found that Geminin maintains a hyperacetylated and open chromatin conformation at neural genes. Interestingly, recombinant Geminin protein also rapidly alters chromatin acetylation and accessibility even when Geminin is combined with nuclear extract and chromatin in vitro. Together, these data support a role for Geminin as a cell intrinsic regulator of neural fate acquisition that promotes expression of neural genes by regulating chromatin accessibility and histone acetylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.