ArticleMolecular microbiology2011
The DNA-binding activity of the Neisseria gonorrhoeae LexA orthologue NG1427 is modulated by oxidation.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- CRISPRi-mediated repression of threeJournal of bacteriology · 2025Article
- Molecular Regulatory Mechanisms Drive Emergent Pathogenetic Properties ofMicroorganisms · 2022Review
- Redox controls RecA protein activity via reversible oxidation of its methionine residues.eLife · 2021Article
- Using rapid point-of-care tests to inform antibiotic choice to mitigate drug resistance in gonorrhoea.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- RNA markers enable phenotypic test of antibiotic susceptibility in Neisseria gonorrhoeae after 10 minutes of ciprofloxacin exposure.Scientific reports · 2018Article
- Activation of σ20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Deciphering the Function of New Gonococcal Vaccine Antigens Using Phenotypic Microarrays.Journal of bacteriology · 2017Article
- Article
- Analyzing Neisseria gonorrhoeae Pilin Antigenic Variation Using 454 Sequencing Technology.Journal of bacteriology · 2016Article
- GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide.Scientific reports · 2016Article
- Molecular Insights into the Impact of Oxidative Stress on the Quorum-Sensing Regulator Protein LasR.The Journal of biological chemistry · 2016Article
- The induction of two biosynthetic enzymes helps Escherichia coli sustain heme synthesis and activate catalase during hydrogen peroxide stress.Molecular microbiology · 2015Article
- DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks.Cold Spring Harbor perspectives in biology · 2013Review
- Article
- Resistance of Neisseria gonorrhoeae to neutrophils.Frontiers in microbiology · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neisseria gonorrhoeae is a human-specific organism that is not usually exposed to UV light or chemicals but is likely to encounter reactive oxygen species during infection. Exposure of N. gonorrhoeae to sublethal hydrogen peroxide revealed that the ng1427 gene was upregulated sixfold. N. gonorrhoeae was thought to lack an SOS system, although NG1427 shows amino acid sequence similarity to the SOS response regulator LexA from Escherichia coli. Similar to LexA and other S24 peptidases, NG1427 undergoes autoproteolysis in vitro, which is facilitated by either the gonococcal or E. coli RecA proteins or high pH, and autoproteolysis requires the active and cleavage site residues conserved between LexA and NG1427. NG1427 controls a three gene regulon: itself; ng1428, a Neisseria-specific, putative integral membrane protein; and recN, a DNA repair gene known to be required for oxidative damage survival. Full NG1427 regulon de-repression requires RecA following methyl methanesulphonate or mitomycin C treatment, but is largely RecA-independent following hydrogen peroxide treatment. NG1427 binds specifically to the operator regions of the genes it controls, and DNA binding is abolished by oxidation of the single cysteine residue encoded in NG1427. We propose that NG1427 is inactivated independently of RecA by oxidation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.