ArticleBiotechnology journal2011
Picoliter DNA sequencing chemistry on an electrowetting-based digital microfluidic platform.
Article in Biotechnology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 69 citations in OpenAlex.
- Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.Lab on a chip · 2026Review
- Digital Microfluidics for Sample Preparation in Low-Input Proteomics.Small methods · 2025Review
- A Deep Reinforcement Learning Approach to Droplet Routing for Erroneous Digital Microfluidic Biochips.Sensors (Basel, Switzerland) · 2023Article
- A comprehensive review on advancements in tissue engineering and microfluidics toward kidney-on-chip.Biomicrofluidics · 2022Review
- Drop-to-drop liquid-liquid extraction of DNA in an electrowetting-on-dielectric digital microfluidics.Biomicrofluidics · 2021Article
- An efficient module-less synthesis approach for Digital Microfluidic Biochip.SN applied sciences · 2020Article
- Digital Microfluidics for Nucleic Acid Amplification.Sensors (Basel, Switzerland) · 2017Review
- Synthesis and cell-free cloning of DNA libraries using programmable microfluidics.Nucleic acids research · 2016Article
- Droplet microfluidics for chip-based diagnostics.Sensors (Basel, Switzerland) · 2014Article
- Topography-assisted electromagnetic platform for blood-to-PCR in a droplet.Biosensors & bioelectronics · 2013Article
- Low voltage picoliter droplet manipulation utilizing electrowetting-on-dielectric platforms.Sensors and actuators. B, Chemical · 2012Article
- Droplet-based pyrosequencing using digital microfluidics.Analytical chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The results of investigations into performing DNA sequencing chemistry on a picoliter-scale electrowetting digital microfluidic platform are reported. Pyrosequencing utilizes pyrophosphate produced during nucleotide base addition to initiate a process ending with detection through a chemiluminescence reaction using firefly luciferase. The intensity of light produced during the reaction can be quantified to determine the number of bases added to the DNA strand. The logic-based control and discrete fluid droplets of a digital microfluidic device lend themselves well to the pyrosequencing process. Bead-bound DNA is magnetically held in a single location, and wash or reagent droplets added or split from it to circumvent product dilution. Here we discuss the dispensing, control, and magnetic manipulation of the paramagnetic beads used to hold target DNA. We also demonstrate and characterize the picoliter-scale reaction of luciferase with adenosine triphosphate to represent the detection steps of pyrosequencing and all necessary alterations for working on this scale.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.