ArticleJournal of artificial organs : the official journal of the Japanese Society for Artificial Organs2011
Induction of putative stratified epithelial progenitor cells in vitro from mouse-induced pluripotent stem cells.
Article in Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Clinical Applications of Corneal Cells Derived from Induced Pluripotent Stem Cells.Biomolecules · 2025Review
- Transdifferentiation of rat keratinocyte progenitors to corneal epithelial cells by limbal niche via the STAT3/PI3K/AKT signaling pathway.Stem cell research & therapy · 2025Article
- Anti-fibrotic effect of a selective estrogen receptor modulator in systemic sclerosis.Stem cell research & therapy · 2022Article
- Bone Morphogenetic Protein 4 (BMP4) Enhances the Differentiation of Human Induced Pluripotent Stem Cells into Limbal Progenitor Cells.Current issues in molecular biology · 2021Article
- Optimization of culture conditions for the efficient differentiation of mouse-induced pluripotent stem cells into dental epithelial-like cells.In vitro cellular & developmental biology. Animal · 2020Article
- Differentiation of mouse-induced pluripotent stem cells into dental epithelial-like cells in the absence of added serum.In vitro cellular & developmental biology. Animal · 2019Article
- Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells.Stem cell research & therapy · 2018Article
- Recent advances of in vitro culture systems for spermatogonial stem cells in mammals.Reproductive medicine and biology · 2018Review
- Article
- Differentiation of human embryonic stem cells into corneal epithelial progenitor cells under defined conditions.PloS one · 2017Article
- Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells.Oncotarget · 2016Article
- Progress in corneal wound healing.Progress in retinal and eye research · 2015Review
- Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.Medical molecular morphology · 2015Article
- Small-molecule induction promotes corneal epithelial cell differentiation from human induced pluripotent stem cells.Stem cell reports · 2014Article
- In vitro epithelial differentiation of human induced pluripotent stem cells for vocal fold tissue engineering.The Annals of otology, rhinology, and laryngology · 2013Article
- Current progress of skin tissue engineering: Seed cells, bioscaffolds, and construction strategies.Burns & trauma · 2013Article
- Journal of Artificial Organs 2011: the year in review.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2012Review
- Article
- Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells.PloS one · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induced pluripotent stem (iPS) cells generally exhibit a normal karyotype, are transcriptionally and epigenetically similar to embryonic stem (ES) cells, and maintain the potential to differentiate into derivatives of all germ layers. Recently, the use of different types of cell or tissue derived from iPS cells for transplantation has become a possibility. However, the differentiation of epithelial lineages from iPS cells has not yet been demonstrated. We attempted to establish a culture technique for the induction of epithelial progenitors from mouse iPS cells. Mouse iPS cells were cultured on dishes coated with type IV collagen in keratinocyte culture medium (KCM) supplemented with or without bone morphogenic protein-4 (BMP-4) or combined with pretreatment of retinoic acid (RA) and BMP-4 in the undifferentiated state. Markers for undifferentiated cells (Oct3/4, Nanog) and for differentiation (p63, cytokeratin14) were analyzed by immunofluorescence staining and real-time RT-PCR. Putative epithelial progenitors were successfully induced in vitro from iPS cells. These progenitors expressed p63, a transcription factor necessary for maintenance of regenerative epithelia and cytokeratin 14 constitutively present in the basal layer of stratified epithelia. Enhancement of putative epithelial progenitor commitment was observed when cultured in KCM with BMP-4 following pretreatment of RA and BMP-4. The differentiation efficiency of putative epithelial progenitors from iPS cell cultures was similar to that of ES cell cultures. This report is the first to demonstrate in vitro differentiation of iPS cells into putative epithelial progenitors. These iPS-derived putative epithelial progenitors provide a powerful tool for understanding the mechanisms of epithelial lineage differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.