ArticleIntegrative biology : quantitative biosciences from nano to macro2011
Subcellular spatial segregation of integrin subtypes by patterned multicomponent surfaces.
Article in Integrative biology : quantitative biosciences from nano to macro, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
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- Cell-cell contact and matrix adhesion promote αSMA expression during TGFβ1-induced epithelial-myofibroblast transition via Notch and MRTF-A.Scientific reports · 2016Article
- Motile Human Neutrophils Sense Ligand Density Over Their Entire Contact Area.Annals of biomedical engineering · 2016Article
- Substrate engagement of integrins α5β1 and αvβ3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin.Scientific reports · 2016Article
- Protrusive and Contractile Forces of Spreading Human Neutrophils.Biophysical journal · 2015Article
- Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins.Biomaterials · 2015Article
- Two-dimensional motility of a macrophage cell line on microcontact-printed fibronectin.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Integrated micro/nanoengineered functional biomaterials for cell mechanics and mechanobiology: a materials perspective.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- Cross talk between CD3 and CD28 is spatially modulated by protein lateral mobility.Molecular and cellular biology · 2014Article
- Ligand density elicits a phenotypic switch in human neutrophils.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Micropatterned multicolor dynamically adhesive substrates to control cell adhesion and multicellular organization.Langmuir : the ACS journal of surfaces and colloids · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
While it is well known that individual integrins are critical mediators of cell behavior, recent work has shown that when multiple types of integrins simultaneously engage the ECM, cell functions are enhanced. However, it is not known how integrins spatially coordinate to regulate cell adhesion because no reliable method exists to segregate integrins on the cell membrane. Here, we use a microcontact printing-based strategy to pattern multiple ECMs that bind distinct integrins in order to study how integrins might interact. In our technique, proteins are first adsorbed uniformly to a poly(dimethyl siloxane) stamp, and then selectively "de-inked." Our strategy overcomes several inherent limitations of conventional microcontact printing, including stamp collapse and limited functionality of the surface patterns. We show that integrins spatially segregate on surfaces patterned with multiple ECMs, as expected. Interestingly, despite spatial segregation of distinct integrins, cells could form adhesions and migrate across multicomponent surfaces as well as they do on single component surfaces. Together, our data indicate that although cells can segregate individual integrins on the cell surface to mediate ECM-specific binding, integrins function cooperatively to guide cell adhesion and migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.