ArticlePloS one2011
A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- EruA, a Regulator of Adherent-InvasiveBiomolecules · 2026Article
- Hyperpiliation, not loss of pilus retraction, reducesMicrobiology spectrum · 2025Article
- Effect ofInternational journal of microbiology · 2025Article
- Contribution of Toxin-Antitoxin Systems to Adherent-InvasiveMicroorganisms · 2024Review
- Article
- Wisdom of the crowds: A suggested polygenic plan for small-RNA-mediated regulation in bacteria.iScience · 2021Article
- Adherent-Invasive and Non-InvasiveMicroorganisms · 2021Article
- Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity.Frontiers in cellular and infection microbiology · 2021Review
- Crc Regulates Succinate-Mediated Repression of Mineral Phosphate Solubilization inFrontiers in microbiology · 2021Article
- The role of major virulence factors and pathogenicity of adherent-invasivePrzeglad gastroenterologiczny · 2020Review
- Replication of Crohn's Disease MucosalPathogens (Basel, Switzerland) · 2019Article
- The RNase YbeY Is Vital for Ribosome Maturation, Stress Resistance, and Virulence of the Natural Genetic EngineerJournal of bacteriology · 2019Article
- A multi-omic analysis reveals the role of fumarate in regulating the virulence of enterohemorrhagic Escherichia coli.Cell death & disease · 2018Article
- Evolutionarily conserved TRH neuropeptide pathway regulates growth inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Functional Characterization ofFrontiers in microbiology · 2017Article
- The Small RNA GcvB Promotes Mutagenic Break Repair by Opposing the Membrane Stress Response.Journal of bacteriology · 2016Article
- sRNA-Mediated Regulation of P-Fimbriae Phase Variation in Uropathogenic Escherichia coli.PLoS pathogens · 2015Article
- Hfq plays important roles in virulence and stress adaptation in Cronobacter sakazakii ATCC 29544.Infection and immunity · 2015Article
- Escherichia coli in chronic inflammatory bowel diseases: An update on adherent invasive Escherichia coli pathogenicity.World journal of gastrointestinal pathophysiology · 2014Review
- Escherichia coli-host macrophage interactions in the pathogenesis of inflammatory bowel disease.World journal of gastroenterology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adherent-invasive Escherichia coli (AIEC) has been linked with the onset and perpetuation of inflammatory bowel diseases. The AIEC strain LF82 was originally isolated from an ileal biopsy from a patient with Crohn's disease. The pathogenesis of LF82 results from its abnormal adherence to and subsequent invasion of the intestinal epithelium coupled with its ability to survive phagocytosis by macrophages once it has crossed the intestinal barrier. To gain further insight into AIEC pathogenesis we employed the nematode Caenorhabditis elegans as an in vivo infection model. We demonstrate that AIEC strain LF82 forms a persistent infection in C. elegans, thereby reducing the host lifespan significantly. This host killing phenotype was associated with massive bacterial colonization of the nematode intestine and damage to the intestinal epithelial surface. C. elegans killing was independent of known LF82 virulence determinants but was abolished by deletion of the LF82 hfq gene, which encodes an RNA chaperone involved in mediating posttranscriptional gene regulation by small non-coding RNAs. This finding reveals that important aspects of LF82 pathogenesis are controlled at the posttranscriptional level by riboregulation. The role of Hfq in LF82 virulence was independent of its function in regulating RpoS and RpoE activity. Further, LF82Δhfq mutants were non-motile, impaired in cell invasion and highly sensitive to various chemical stress conditions, reinforcing the multifaceted function of Hfq in mediating bacterial adaptation. This study highlights the usefulness of simple non-mammalian infection systems for the identification and analysis of bacterial virulence factors.
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