ArticlePloS one2011
High-resolution melting system to perform multilocus sequence typing of Campylobacter jejuni.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars ofVeterinary sciences · 2026Article
- Development of a Multi-Locus Real-Time PCR with a High-Resolution Melting Assay to Differentiate Wild-Type, Asian Recombinant, and Vaccine Strains of Lumpy Skin Disease Virus.Veterinary sciences · 2025Article
- Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis.Microbiology spectrum · 2023Article
- Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization.Microbiology spectrum · 2022Article
- Molecular typing of Campylobacter jejuni strains: comparison among four different techniques.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China.Current microbiology · 2015Article
- Development of a comparative genomic fingerprinting assay for rapid and high resolution genotyping of Arcobacter butzleri.BMC microbiology · 2015Article
- Article
- Differentiation of Campylobacter jejuni and Campylobacter coli Using Multiplex-PCR and High Resolution Melt Curve Analysis.PloS one · 2015Article
- Multicolor Melting Curve Analysis-Based Multilocus Melt Typing of Vibrio parahaemolyticus.PloS one · 2015Article
- Genotyping ofBiotechnology, biotechnological equipment · 2014Article
- Article
- Use of amplified-fragment length polymorphism to study the ecology of Campylobacter jejuni in environmental water and to predict multilocus sequence typing clonal complexes.Applied and environmental microbiology · 2012Article
- Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth.BMC research notes · 2012Article
- Article
- Application of high-resolution DNA melting for genotyping in lepidopteran non-model species: Ostrinia furnacalis (Crambidae).PloS one · 2012Article
- High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex.Journal of clinical microbiology · 2011Article
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4 authors.
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Abstract
Multi-locus sequence typing (MLST) has emerged as the state-of-the-art method for resolving bacterial population genetics but it is expensive and time consuming. We evaluated the potential of high resolution melting (HRM) to identify known MLST alleles of Campylobacter jejuni at reduced cost and time. Each MLST locus was amplified in two or three sub fragments, which were analyzed by HRM. The approach was investigated using 47 C. jejuni isolates, previously characterized by classical MLST, representing isolates from diverse environmental, animal and clinical sources and including the six most prevalent sequence types (ST) and the most frequent alleles. HRM was then applied to a validation set of 84 additional C. jejuni isolates from chickens; 92% of the alleles were resolved in 35 hours of laboratory time and the cost of reagents per isolate was $20 compared with $100 for sequence-based typing. HRM has the potential to complement sequence-based methods for resolving SNPs and to facilitate a wide range of genotyping studies.
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