ArticleNature methods2011
High-throughput single-molecule optofluidic analysis.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed.
- Single-Molecule Forster Resonance Energy Transfer With a Minimalistic 3D-Printed Setup and Dyes in the Blue-Green Spectral Region.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Single-molecule digital sizing of proteins in solution.Nature communications · 2024Article
- A review on biodiesel micromixers: Types of micromixers, configurations, and flow patterns.Heliyon · 2024Review
- Evidence for a compact σiScience · 2024Article
- An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics.Nature communications · 2023Article
- Towards an active droplet-based microfluidic platform for programmable fluid handling.Lab on a chip · 2023Article
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- The structural heterogeneity of α-synuclein is governed by several distinct subpopulations with interconversion times slower than milliseconds.Structure (London, England : 1993) · 2021Article
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Machine-driven parameter screen of biochemical reactions.Nucleic acids research · 2020Article
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Systematic Assessment of Burst Impurity in Confocal-Based Single-Molecule Fluorescence Detection Using Brownian Motion Simulations.Molecules (Basel, Switzerland) · 2019Article
- The effect of macromolecular crowding on single-round transcription by Escherichia coli RNA polymerase.Nucleic acids research · 2019Article
- Controller for microfluidic large-scale integration.HardwareX · 2018Article
- Characterizing highly dynamic conformational states: The transcription bubble in RNAP-promoter open complex as an example.The Journal of chemical physics · 2018Article
- Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer.Science (New York, N.Y.) · 2018Review
- AnThe Journal of biological chemistry · 2017Article
- Different types of pausing modes during transcription initiation.Transcription · 2017Article
- High-throughput automated microfluidic sample preparation for accurate microbial genomics.Nature communications · 2017Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
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Authors and funding
6 authors.
Funding
Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.