ArticleNucleic acids research2011
Thermophoretic melting curves quantify the conformation and stability of RNA and DNA.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Incorporation of NUPACK-Based Simulation into Classroom and Laboratory Teaching of Nucleic Acids Hybridization for Undergraduate Biochemistry.Journal of chemical education · 2025Article
- The Last Universal Common Ancestor of Ribosome-Encoding Organisms: Portrait of LUCA.Journal of molecular evolution · 2024Review
- RNAs undergo phase transitions with lower critical solution temperatures.Nature chemistry · 2023Article
- Stability of End-to-End Base Stacking Interactions in Highly Concentrated DNA Solutions.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Nucleic acid nanostructures forApplied physics reviews · 2023Review
- Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics.Angewandte Chemie (International ed. in English) · 2021Article
- Temperature-Responsive Peptide-Nucleotide Coacervates.The journal of physical chemistry. B · 2021Article
- Topoisomerases inhibition and DNA binding mode of daunomycin-oligoarginine conjugate.Journal of enzyme inhibition and medicinal chemistry · 2020Article
- Thermophoresis: The Case of Streptavidin and Biotin.Polymers · 2020Article
- Unveiling the druggable RNA targets and small molecule therapeutics.Bioorganic & medicinal chemistry · 2019Review
- Increased levels of the long noncoding RNA, HOXA-AS3, promote proliferation of A549 cells.Cell death & disease · 2018Article
- Berberine ameliorates non-alcoholic steatohepatitis in ApoEExperimental and therapeutic medicine · 2017Article
- Hepatitis C virus double-stranded RNA is the predominant form in human liver and in interferon-treated cells.Hepatology (Baltimore, Md.) · 2017Article
- The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- N(6)-Methyladenosine: a conformational marker that regulates the substrate specificity of human demethylases FTO and ALKBH5.Scientific reports · 2016Article
- Complicated behavior of G-quadruplexes and evaluating G-quadruplexes' ligands in various systems mimicking cellular circumstance.Biochemistry and biophysics reports · 2016Article
- Article
- A novel phosphorylation-independent interaction between SMG6 and UPF1 is essential for human NMD.Nucleic acids research · 2014Article
- Accurate zygote-specific discrimination of single-nucleotide polymorphisms using microfluidic electrochemical DNA melting curves.Angewandte Chemie (International ed. in English) · 2014Article
- RNAtips: Analysis of temperature-induced changes of RNA secondary structure.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring parameters such as stability and conformation of biomolecules, especially of nucleic acids, is important in the field of biology, medical diagnostics and biotechnology. We present a thermophoretic method to analyse the conformation and thermal stability of nucleic acids. It relies on the directed movement of molecules in a temperature gradient that depends on surface characteristics of the molecule, such as size, charge and hydrophobicity. By measuring thermophoresis of nucleic acids over temperature, we find clear melting transitions and resolve intermediate conformational states. These intermediate states are indicated by an additional peak in the thermophoretic signal preceding most melting transitions. We analysed single nucleotide polymorphisms, DNA modifications, conformational states of DNA hairpins and microRNA duplexes. The method is validated successfully against calculated melting temperatures and UV absorbance measurements. Interestingly, the methylation of DNA is detected by the thermophoretic amplitude even if it does not affect the melting temperature. In the described setup, thermophoresis is measured all-optical in a simple setup using a reproducible capillary format with only 250 nl probe consumption. The thermophoretic analysis of nucleic acids shows the technique's versatility for the investigation of nucleic acids relevant in cellular processes like RNA interference or gene silencing.
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