ArticleNucleic acids research2011
Molecular breeding of polymerases for resistance to environmental inhibitors.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Rapid evolution of a highly efficient RNA polymerase by homologous recombination.Nature chemical biology · 2026Article
- Counting soil microbial communities: the impact of qPCR platform and mastermix on accuracy and precision.FEMS microbiology ecology · 2025Article
- Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors.Frontiers in bioengineering and biotechnology · 2025Article
- Development of Single Molecule Techniques for Sensing and Manipulation of CRISPR and Polymerase Enzymes.Small (Weinheim an der Bergstrasse, Germany) · 2023Review
- DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.International journal of molecular sciences · 2023Review
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- Bacteria and Yeast Colony PCR.Methods in molecular biology (Clifton, N.J.) · 2023Article
- PCR inhibition in qPCR, dPCR and MPS-mechanisms and solutions.Analytical and bioanalytical chemistry · 2020Review
- Subduing the influence of PCR inhibitors on amplifying aged, degraded, and low copy number DNA: PCR enhancer cocktail-p and rescue PCR.PloS one · 2020Article
- Engineering Polymerases for New Functions.Trends in biotechnology · 2019Review
- Engineering of a thermostable viral polymerase using metagenome-derived diversity for highly sensitive and specific RT-PCR.Nucleic acids research · 2019Article
- Extended persistence of general and cattle-associated fecal indicators in marine and freshwater environment.The Science of the total environment · 2019Article
- Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library.Scientific reports · 2019Article
- Essential role of polymerases for assay performance - Impact of polymerase replacement in a well-established assay.Biomolecular detection and quantification · 2018Article
- Detection ofThe American journal of tropical medicine and hygiene · 2018Article
- Improved Detection of Norovirus and Hepatitis A Virus in Surface Water by Applying Pre-PCR Processing.Food and environmental virology · 2017Article
- Efficient isolation method for high-quality genomic DNA from cicada exuviae.Ecology and evolution · 2017Article
- Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase.Nucleic acids research · 2017Article
- Exploring the Chemistry of Genetic Information Storage and Propagation through Polymerase Engineering.Accounts of chemical research · 2017Review
- Necessity of purification during bacterial DNA extraction with environmental soils.Environmental health and toxicology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
Abstract
Potent inhibitors limit the use of PCR assays in a wide spectrum of specimens. Here, we describe the engineering of polymerases with a broad resistance to complex environmental inhibitors using molecular breeding of eight different polymerase orthologues from the genus Thermus and directed evolution by CSR in the presence of inhibitors. Selecting for resistance to the inhibitory effects of Neomylodon bone powder, we isolated 2D9, a chimeric polymerase comprising sequence elements derived from DNA polymerases from Thermus aquaticus, Thermus oshimai, Thermus thermophilus and Thermus brockianus. 2D9 displayed a striking resistance to a broad spectrum of complex inhibitors of highly divergent composition including humic acid, bone dust, coprolite, peat extract, clay-rich soil, cave sediment and tar. The selected polymerase promises to have utility in PCR-based applications in a wide range of fields including palaeobiology, archaeology, conservation biology, forensic and historic medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.