ArticleJournal of bacteriology2011
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
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- Whole genome analysis of Pseudomonas mandelii SW-3 and the insights into low-temperature adaptation.Folia microbiologica · 2024Article
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- Investigation of cold-resistance mechanisms in cryophylactic yeastFrontiers in microbiology · 2024Article
- Efficient low-temperature wastewater treatment byFrontiers in microbiology · 2024Article
- Comparative genomic analysis of two Arctic Pseudomonas strains reveals insights into the aerobic denitrification in cold environments.BMC genomics · 2023Article
- Morphological and physiological adaptations of psychrophilic Pseudarthrobacter psychrotolerans YJ56 under temperature stress.Scientific reports · 2023Article
- Transcriptome-wide probing reveals RNA thermometers that regulate translation of glycerol permease genes inRNA (New York, N.Y.) · 2023Article
- Dynamic Transcriptional Landscape ofInternational journal of molecular sciences · 2023Article
- Effect of Selected Factors Influencing Biogenic Amines Degradation byMicroorganisms · 2023Article
- EngineeringFrontiers in bioengineering and biotechnology · 2023Article
- Mining of key genes for cold adaptation fromFrontiers in microbiology · 2023Article
- Complete genome of Nakamurella sp. PAMC28650: genomic insights into its environmental adaptation and biotechnological potential.Functional & integrative genomics · 2022Article
16 more citing papers are in PubMed but not listed here.
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Accumulation of compatible solutes is a strategy widely employed by bacteria to achieve cellular protection against high osmolarity. These compounds are also used in some microorganisms as thermostress protectants. We found that Bacillus subtilis uses the compatible solute glycine betaine as an effective cold stress protectant. Glycine betaine strongly stimulated growth at 15°C and permitted cell proliferation at the growth-inhibiting temperature of 13°C. Initial uptake of glycine betaine at 15°C was low but led eventually to the buildup of an intracellular pool whose size was double that found in cells grown at 35°C. Each of the three glycine betaine transporters (OpuA, OpuC, and OpuD) contributed to glycine betaine accumulation in the cold. Protection against cold stress was also accomplished when glycine betaine was synthesized from its precursor choline. Growth of a mutant defective in the osmoadaptive biosynthesis for the compatible solute proline was not impaired at low temperature (15°C). In addition to glycine betaine, the compatible solutes and osmoprotectants l-carnitine, crotonobetaine, butyrobetaine, homobetaine, dimethylsulfonioactetate, and proline betaine all served as cold stress protectants as well and were accumulated via known Opu transport systems. In contrast, the compatible solutes and osmoprotectants choline-O-sulfate, ectoine, proline, and glutamate were not cold protective. Our data highlight an underappreciated facet of the acclimatization of B. subtilis to cold environments and allow a comparison of the characteristics of compatible solutes with respect to their osmotic, heat, and cold stress-protective properties for B. subtilis cells.
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