Evidence map›Paper›PMID 21296953›Full record

ArticleApplied and environmental microbiology2011

Efficient generation of unmarked deletions in Legionella pneumophila.

Andrew Bryan, Kaoru Harada, Michele S Swanson

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
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  9. The Toolbox for Uncovering the Functions ofFrontiers in cellular and infection microbiology · 2017
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Andrew BryanDepartment of Microbiology and Immunology, University of Michigan Medical School, 5641 Medical Sciences Building II, 1150 West Medical Center Dr., Ann Arbor, MI 48109-5620, USA.
Kaoru Harada
Michele S Swanson

Funding

MICHIGAN MEDICAL SCIENTIST TRAINING PROGRAMT32GM007863 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI COLLINS, KATHLEEN L. · 1985 to 2024
$38.3M
Molecular Mechanisms of Microbial Pathogenesis Training ProgramT32AI007528 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI CARRUTHERS, VERNON BRUCE · 1998 to 2024
$6.8M
Analysis of L. Pneumophilia Virulence RegulationR01AI044212 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SWANSON, MICHELE SOMES · 1999 to 2010
$2.1M
NIAID NIH HHS AI044212-09S1NIAID NIH HHS R01 AI044212NIAID NIH HHS T32 AI007528NIAID NIH HHS T32 AI07528-10NIGMS NIH HHS T32 GM007863NIGMS NIH HHS T32 GM07863
6 · The paper itself

Abstract

Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using λ-Red recombinase were transferred to L. pneumophila by natural transformation. Resistance cassettes were then efficiently excised using the Flp site-specific recombinase encoded on a plasmid that is readily lost.

Indexed as

Sequence DeletionEscherichia coliGenetics, MicrobialLegionella pneumophilaMutagenesis, InsertionalPlasmidsRecombination, GeneticSelection, GeneticTransformation, Bacterial

Identifiers

PMID21296953
PMCPMC3067435

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.