ArticleApplied and environmental microbiology2011
Transcriptome, proteome, and metabolite analyses of a lactate dehydrogenase-negative mutant of Enterococcus faecalis V583.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Purification and characterization of bacteriocins-like inhibitory substances from food isolated Enterococcus faecalis OS13 with activity against nosocomial enterococci.Scientific reports · 2021Article
- Transcriptome Analysis Reveals Catabolite Control Protein A Regulatory Mechanisms Underlying Glucose-Excess or -Limited Conditions in a Ruminal Bacterium,Frontiers in microbiology · 2021Article
- Single-Nucleotide RNA Maps for the Two Major Nosocomial PathogensFrontiers in cellular and infection microbiology · 2020Article
- The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response ofFrontiers in cellular and infection microbiology · 2018Article
- NADH-Mediated Gene Expression inFrontiers in microbiology · 2018Article
- Effects of glucose availability in Lactobacillus sakei; metabolic change and regulation of the proteome and transcriptome.PloS one · 2017Article
- Hyaluronan production and molecular weight is enhanced in pathway-engineered strains of lactate dehydrogenase-deficientMetabolic engineering communications · 2016Article
- Transcription factor Rex in regulation of pathophysiology in oral pathogens.Molecular oral microbiology · 2016Review
- Regulating the Intersection of Metabolism and Pathogenesis in Gram-positive Bacteria.Microbiology spectrum · 2015Review
- Whole-genome mapping of 5' RNA ends in bacteria by tagged sequencing: a comprehensive view in Enterococcus faecalis.RNA (New York, N.Y.) · 2015Article
- Using a genome-scale metabolic model of Enterococcus faecalis V583 to assess amino acid uptake and its impact on central metabolism.Applied and environmental microbiology · 2015Article
- Enterococcus faecalis grows on ascorbic acid.Applied and environmental microbiology · 2013Article
- Redox balance via lactate dehydrogenase is important for multiple stress resistance and virulence in Enterococcus faecalis.Infection and immunity · 2013Article
- A Rex family transcriptional repressor influences H2O2 accumulation by Enterococcus faecalis.Journal of bacteriology · 2013Article
- Characterization of a Leuconostoc bacteriophage infecting flavor producers of cheese starter cultures.Applied and environmental microbiology · 2012Article
- Growth rate-dependent control in Enterococcus faecalis: effects on the transcriptome and proteome, and strong regulation of lactate dehydrogenase.Applied and environmental microbiology · 2012Article
- Genome-wide transcriptome and proteome analysis on different developmental stages of Cordyceps militaris.PloS one · 2012Article
- Article
- CcpA represses the expression of the divergent cit operons of Enterococcus faecalis through multiple cre sites.BMC microbiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A constructed lactate dehydrogenase (LDH)-negative mutant of Enterococcus faecalis V583 grows at the same rate as the wild type but ferments glucose to ethanol, formate, and acetoin. Microarray analysis showed that LDH deficiency had profound transcriptional effects: 43 genes in the mutant were found to be upregulated, and 45 were found to be downregulated. Most of the upregulated genes encode enzymes of energy metabolism or transport. By two-dimensional (2D) gel analysis, 45 differentially expressed proteins were identified. A comparison of transcriptomic and proteomic data suggested that for several proteins the level of expression is regulated beyond the level of transcription. Pyruvate catabolic genes, including the truncated ldh gene, showed highly increased transcription in the mutant. These genes, along with a number of other differentially expressed genes, are preceded by sequences with homology to binding sites for the global redox-sensing repressor, Rex, of Staphylococcus aureus. The data indicate that the genes are transcriptionally regulated by the NADH/NAD ratio and that this ratio plays an important role in the regulatory network controlling energy metabolism in E. faecalis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.