ArticleNucleic acids research2011
NSrp70 is a novel nuclear speckle-related protein that modulates alternative pre-mRNA splicing in vivo.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- The association between miR-423 rs6505162 polymorphism and cancer susceptibility: a systematic review and meta-analysis.Oncotarget · 2017Pooled it
- Integrative Genotyping and Analysis of Canine Structural Variation Using Long-read and Short-read Data.Genome biology and evolution · 2025Article
- RNA-binding proteins regulating the CD44 alternative splicing.Frontiers in molecular biosciences · 2023Review
- Influence of Micro-RNA-423 Gene Variation on Risk and Characteristics of Breast Cancer.Asian Pacific journal of cancer prevention : APJCP · 2022Article
- NSrp70 suppresses metastasis in triple-negative breast cancer by modulating Numb/TβR1/EMT axis.Oncogene · 2022Article
- Additive and Dominance Genomic Analysis for Litter Size in Purebred and Crossbred Iberian Pigs.Genes · 2021Article
- Biallelic loss-of-function variants in the splicing regulator NSRP1 cause a severe neurodevelopmental disorder with spastic cerebral palsy and epilepsy.Genetics in medicine : official journal of the American College of Medical Genetics · 2021Article
- CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.Nucleic acids research · 2021Article
- NSrp70 is a lymphocyte-essential splicing factor that controls thymocyte development.Nucleic acids research · 2021Article
- Article
- Rbm14 maintains the integrity of genomic DNA during early mouse embryogenesis via mediating alternative splicing.Cell proliferation · 2020Article
- New PCNT candidate missense variant in a patient with oral and maxillofacial osteodysplasia: a case report.BMC medical genetics · 2019Article
- Involvement of microRNA-423 Gene Variability in Breast Cancer Progression in Saudi ArabiaAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Minor zygotic gene activation is essential for mouse preimplantation development.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Comparison of village dog and wolf genomes highlights the role of the neural crest in dog domestication.BMC biology · 2018Article
- Proteome-wide Mapping of Endogenous SUMOylation Sites in Mouse Testis.Molecular & cellular proteomics : MCP · 2017Article
- Splicing in immune cells-mechanistic insights and emerging topics.International immunology · 2017Review
- Nuclear Speckle-related Protein 70 Binds to Serine/Arginine-rich Splicing Factors 1 and 2 via an Arginine/Serine-like Region and Counteracts Their Alternative Splicing Activity.The Journal of biological chemistry · 2016Article
- The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing.The EMBO journal · 2015Article
- Identification of miR-423 and miR-499 polymorphisms on affecting the risk of hepatocellular carcinoma in a large-scale population.Genetic testing and molecular biomarkers · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear speckles are known to be the storage sites of mRNA splicing regulators. We report here the identification and characterization of a novel speckle protein, referred to as NSrp70, based on its subcellular localization and apparent molecular weight. This protein was first identified as CCDC55 by the National Institutes of Health Mammalian Gene Collection, although its function has not been assigned. NSrp70 was colocalized and physically interacted with SC35 and ASF/SF2 in speckles. NSrp70 has a putative RNA recognition motif, the RS-like region, and two coiled-coil domains, suggesting a role in RNA processing. Accordingly, using CD44, Tra2β1 and Fas constructs as splicing reporter minigenes, we found that NSrp70 modulated alternative splice site selection in vivo. The C-terminal 10 amino acids (531-540), including (536)RD(537), were identified as a novel nuclear localization signal, and the region spanning 290-471 amino acids was critical for speckle localization and binding to SC35 and ASF/SF2. The N-terminal region (107-161) was essential for the pre-mRNA splicing activity. Finally, we found that knockout of NSrp70 gene in mice led to a lack of progeny, including fetal embryos. Collectively, we demonstrate that NSrp70 is a novel splicing regulator and essentially required early stage of embryonic development.
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