Evidence map›Paper›PMID 21296083›Full record

ReviewJournal of immunological methods2011

Methodology and application of flow cytometry for investigation of human malaria parasites.

Brian T Grimberg

Abstract readReview
In one paragraph

Review in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

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  20. Disruption of theACS infectious diseases · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Brian T GrimbergCenter for Global Health and Diseases, Case Western Reserve, University, Wolstein Research Building, 4-134 Cleveland, OH 44106-7286, United States. endmalaria@case.edu

Funding

TUMOR METABOLISM PROGRAMP30CA043703 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI Drew James Adams · 1987 to 2026
$142.3M
Duffy antigen presentation and its correlation to parasite invasionK22AI079388 · NIAID · CASE WESTERN RESERVE UNIVERSITY · PI GRIMBERG, BRIAN T. · 2010 to 2011
$270k
NCI NIH HHS P30 CA043703NCI NIH HHS P30 CA43703NIAID NIH HHS AI079388NIAID NIH HHS K22 AI079388
6 · The paper itself

Abstract

Historically, examinations of the inhibition of malaria parasite growth/invasion, whether using drugs or antibodies, have relied on the use of microscopy or radioactive hypoxanthine uptake. These are considered gold standards for measuring the effectiveness of antimalarial treatments, however, these methods have well known shortcomings. With the advent of flow cytometry coupled with the use of fluorescent DNA stains allowed for increased speed, reproducibility, and qualitative estimates of the effectiveness of antibodies and drugs to limit malaria parasite growth which addresses the challenges of traditional techniques. Because materials and machines available to research facilities are so varied, different methods have been developed to investigate malaria parasites by flow cytometry. This review is intended to serve as a reference guide for advanced users and importantly, as a primer for new users, to support expanded use and improvements to malaria flow cytometry, particularly in endemic countries.

Indexed as

AnimalsColoring AgentsDNA, ProtozoanErythrocytesFlow CytometryHumansLife Cycle StagesParasitemiaPlasmodiumColoring AgentsDNA, Protozoan

Identifiers

PMID21296083
PMCPMC3071436

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.