ArticleSmall (Weinheim an der Bergstrasse, Germany)2011
Ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification in a microfluidic nanoliter platform.
Article in Small (Weinheim an der Bergstrasse, Germany), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Cell-Free Production Systems in Droplet Microfluidics.Advances in biochemical engineering/biotechnology · 2023Review
- Polymeric dipicolylamine based mass tags for mass cytometry.Chemical science · 2022Article
- Recent Advances in Signal Amplification to Improve Electrochemical Biosensing for Infectious Diseases.Frontiers in chemistry · 2022Article
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Lanthanide nanoparticles for high sensitivity multiparameter single cell analysis.Chemical science · 2019Article
- Branched rolling circle amplification method for measuring serum circulating microRNA levels for early breast cancer detection.Cancer science · 2018Article
- Selective detection of N6-methyladenine in DNAChemical science · 2017Article
- Biocompatibility of fluids for multiphase drops-in-drops microfluidics.Biomedical microdevices · 2016Article
- The Discovery of Rolling Circle Amplification and Rolling Circle Transcription.Accounts of chemical research · 2016Article
- Ultrasensitive detection of DNA and protein markers in cancer cells.Cancer biology & medicine · 2015Review
- Development of a low-volume, highly sensitive microimmunoassay using computational fluid dynamics-driven multiobjective optimization.Microfluidics and nanofluidics · 2015Article
- Toward integrated molecular diagnostic system (i MDx): principles and applications.IEEE transactions on bio-medical engineering · 2014Review
- Isolation and amplification of mRNA within a simple microfluidic lab on a chip.Analytical chemistry · 2014Article
- Live single cell functional phenotyping in droplet nano-liter reactors.Scientific reports · 2013Article
- Target DNA detection and quantitation on a single cell with single base resolution.Technology · 2013Article
- Article
- Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cells.Journal of the American Chemical Society · 2012Article
- Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection.Mikrochimica acta · 2012Article
- Micro total analysis systems for cell biology and biochemical assays.Analytical chemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
With advances in immunology and cancer biology, there is an unmet need for increasingly sensitive systems to monitor the expression of specific cell markers for the development of new diagnostic and therapeutic tools. To address this challenge, a highly sensitive labeling method that translates antigen-antibody recognition processes into DNA detection events that can be greatly amplified via isothermal rolling circle amplification (RCA) is applied. By merging the single-molecule detection power of RCA reactions with microfluidic technology, it is demonstrated that the identification of specific protein markers can be achieved on tumor-cell surfaces in miniaturized nanoliter reaction droplets. Furthermore, this combined approach of signal amplification in a microfluidic format could extend the utility of existing methods by reducing sample and reagent consumption and enhancing the sensitivities and specificities for various applications, including early diagnosis of cancer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.