ArticleChemistry (Weinheim an der Bergstrasse, Germany)2011
Two successive reactions on a DNA template: a strategy for improving background fluorescence and specificity in nucleic acid detection.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Bioorthogonal Reactions of Triarylphosphines and Related Analogues.Chemical reviews · 2021Review
- Nucleic Acid Templated Reactions for Chemical Biology.ChemMedChem · 2017Review
- Fluorogenic Templated Reaction Cascades for RNA Detection.Journal of the American Chemical Society · 2017Article
- Bioorthogonal tetrazine-mediated transfer reactions facilitate reaction turnover in nucleic acid-templated detection of microRNA.Journal of the American Chemical Society · 2014Article
- Quantification of plasma HIV RNA using chemically engineered peptide nucleic acids.Nature communications · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Oligonucleotide-templated reactions for sensing nucleic acids.Molecules (Basel, Switzerland) · 2012Review
- Multi-path quenchers: efficient quenching of common fluorophores.Bioconjugate chemistry · 2011Article
- Improved templated fluorogenic probes enhance the analysis of closely related pathogenic bacteria by microscopy and flow cytometry.Bioconjugate chemistry · 2011Article
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Authors and funding
2 authors.
Funding
Abstract
We report a new strategy for template-mediated fluorogenic chemistry that results in enhanced performance for the fluorescence detection of nucleic acids. In this approach, two successive templated reactions are required to induce a fluorescence signal, rather than only one. These novel fluorescein-labeled oligonucleotide probes, termed 2-STAR (STAR = Staudinger-triggered α-azidoether release) probes, contain two quencher groups tethered by separate reductively cleavable linkers. When a 2-STAR quenched probe successively binds adjacent to two mono-triphenylphosphine-(TPP)-DNAs or one dual-TPP-DNA, the two quenchers are released, resulting in a fluorescence signal. Because of the requirement for two consecutive reactions, 2-STAR probes display an unprecedented level of sequence specificity for template-mediated probe designs. At the same time, background emission generated by off-template reactions or incomplete quenching is among the lowest of any fluorogenic reactive probes for the detection of DNA or RNA.
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