ArticleProceedings of the National Academy of Sciences of the United States of America2011
Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Transneuronal Circuit Analysis with Pseudorabies Viruses.Current protocols · 2023Review
- Descending pathways from the superior colliculus mediating autonomic and respiratory effects associated with orienting behaviour.The Journal of physiology · 2022Article
- Sympathoexcitatory responses to renal chemosensitive stimuli are exaggerated at nighttime in rats.American journal of physiology. Heart and circulatory physiology · 2022Article
- Transneuronal tracing to map connectivity in injured and transplanted spinal networks.Experimental neurology · 2022Review
- The connectome from the cerebral cortex to the viscera using viral transneuronal tracers.American journal of translational research · 2021Review
- Anterograde Neuronal Circuit Tracers Derived from Herpes Simplex Virus 1: Development, Application, and Perspectives.International journal of molecular sciences · 2020Review
- Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses.Neuroscience bulletin · 2019Review
- Differences in neurotropism and neurotoxicity among retrograde viral tracers.Molecular neurodegeneration · 2019Article
- Characterization of the neuroinvasive profile of a pseudorabies virus recombinant expressing the mTurquoise2 reporter in single and multiple injection experiments.Journal of neuroscience methods · 2018Article
- Neuroanatomical autonomic substrates of brainstem-gut circuitry identified using transsynaptic tract-tracing with pseudorabies virus recombinants.American journal of clinical and experimental immunology · 2018Article
- Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats.Physiological genomics · 2017Article
- Neuroanatomical circuitry between kidney and rostral elements of brain: a virally mediated transsynaptic tracing study in mice.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2017Article
- Specific behavioral and cellular adaptations induced by chronic morphine are reduced by dietary omega-3 polyunsaturated fatty acids.PloS one · 2017Article
- Mapping and Analysis of the Connectome of Sympathetic Premotor Neurons in the Rostral Ventrolateral Medulla of the Rat Using a Volumetric Brain Atlas.Frontiers in neural circuits · 2017Article
- Afferent and efferent connections of C1 cells with spinal cord or hypothalamic projections in mice.Brain structure & function · 2016Article
- Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model.Oncotarget · 2016Article
- Large-Scale Fluorescence Calcium-Imaging Methods for Studies of Long-Term Memory in Behaving Mammals.Cold Spring Harbor perspectives in biology · 2016Review
- Review
- Distinct brainstem and forebrain circuits receiving tracheal sensory neuron inputs revealed using a novel conditional anterograde transsynaptic viral tracing system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Brainbow: new resources and emerging biological applications for multicolor genetic labeling and analysis.Genetics · 2015Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Transneuronal transport of neurotropic viruses is widely used to define the organization of neural circuitry in the mature and developing nervous system. However, interconnectivity within complex circuits limits the ability of viral tracing to define connections specifically linked to a subpopulation of neurons within a network. Here we demonstrate a unique viral tracing technology that highlights connections to defined populations of neurons within a larger labeled network. This technology was accomplished by constructing a replication-competent strain of pseudorabies virus (PRV-263) that changes the profile of fluorescent reporter expression in the presence of Cre recombinase (Cre). The viral genome carries a Brainbow cassette that expresses a default red reporter in infected cells. However, in the presence of Cre, the red reporter gene is excised from the genome and expression of yellow or cyan reporters is enabled. We used PRV-263 in combination with a unique lentivirus vector that produces Cre expression in catecholamine neurons. Projection-specific infection of central circuits containing these Cre-expressing catecholamine neurons with PRV-263 resulted in Cre-mediated recombination of the PRV-263 genome and conditional expression of cyan/yellow reporters. Replication and transneuronal transport of recombined virus produced conditional reporter expression in neurons synaptically linked to the Cre-expressing catecholamine neurons. This unique technology highlights connections specific to phenotypically defined neurons within larger networks infected by retrograde transneuronal transport of virus from a defined projection target. The availability of other technologies that restrict Cre expression to defined populations of neurons indicates that this approach can be widely applied across functionally defined systems.
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