Evidence map›Paper›PMID 21292859›Full record

ArticleJournal of medical microbiology2011

Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children.

C A Contreras, T J Ochoa, J Ruiz, D W Lacher, F P Rivera, Y Saenz, E Chea-Woo, N Zavaleta, A I Gil, C F Lanata and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Genomic Epidemiology of Shiga Toxin-ProducingAnimals : an open access journal from MDPI · 2021
    Article
  3. Article
  4. Article
  5. Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015
    Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 9 institutions in 3 countries.

C A ContrerasInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
T J OchoaUniversity of Texas School of Public Health, Houston, USA.
J RuizCIBERESP, Barcelona, Spain.
D W LacherUS Food and Drug Administration, Laurel, Maryland, USA.
F P RiveraInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
Y SaenzÁrea de Microbiología Molecular, Centro de Investigación Biomédica La Rioja, Logroño, Spain.
E Chea-WooUniversidad Peruana Cayetano Heredia, Lima, Peru.
N ZavaletaInstituto de Investigación Nutricional, Lima, Peru.
A I GilInstituto de Investigación Nutricional, Lima, Peru.
C F LanataEscuela de Medicina, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
L HuichoInstituto Nacional de Salud del Niño, Lima, Peru.
R C MavesUS Naval Medical Research Center Detachment, Department of Bacteriology, Lima, Peru.
C TorresÁrea de Microbiología Molecular, Centro de Investigación Biomédica La Rioja, Logroño, Spain.
C DebRoyE. coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, Pennsylvania, USA.
T G ClearyUniversity of Texas School of Public Health, Houston, USA.
Universidad Peruana Cayetano Heredia · PECenter for Biomedical Research of La Rioja · ESInstituto de Investigación Nutricional · PEThe University of Texas Health Science Center at Houston · USCentre de Recerca en Economia Internacional · ESNaval Medical Research Unit Six · PEPennsylvania State University · USPeruvian University of Applied Sciences · PEUnited States Food and Drug Administration · US

Funding

Randomonized controlled trial-lactoferrin prevention of diarrhea in childrenR01HD051716 · NICHD · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI CLEARY, THOMAS GEORGE · 2007 to 2010
$960k
Role of virulence heterogeneity in acute and presistent EPEC diarrheaK01TW007405 · FIC · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI OCHOA, THERESA J · 2006 to 2009
$433k
FIC NIH HHS 1K01TW007405FIC NIH HHS K01 TW007405NICHD NIH HHS R01 HD051716NICHD NIH HHS R01-HD051716
6 · The paper itself

Abstract

The aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) and phylogenetic relationships [PFGE and multilocus sequence typing (MLST)] of Shiga toxin-producing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged <36 months. STEC prevalence was 0.4 % (14/3219) in diarrhoeal and 0.6 % (15/2695) in control samples. None of the infected children developed haemolytic uraemic syndrome (HUS) or other complications of STEC. stx1 was present in 83 % of strains, stx2 in 17 %, eae in 72 %, ehxA in 59 % and astA in 14 %. The most common serotype was O26 : H11 (14 %) and the most common seropathotype was B (45 %). The strains belonged mainly to phylogenetic group B1 (52 %). The distinct combinations of alleles across the seven MLST loci were used to define 13 sequence types among 19 STEC strains. PFGE typing of 20 STEC strains resulted in 19 pulsed-field patterns. Comparison of the patterns revealed 11 clusters (I-XI), each usually including strains belonging to different serotypes; one exception was cluster VI, which gathered exclusively seven strains of seropathotype B, clonal group enterohaemorrhagic E. coli (EHEC) 2 and phylogenetic group B1. In summary, STEC prevalence was low in Peruvian children with diarrhoea in the community setting. The strains were phylogenetically diverse and associated with mild infections. However, additional studies are needed in children with bloody diarrhoea and HUS.

Indexed as

Adhesins, BacterialBase SequenceCase-Control StudiesChild, PreschoolCohort StudiesDNA, BacterialElectrophoresis, Gel, Pulsed-FieldEscherichia coli InfectionsEscherichia coli ProteinsFemaleGenes, BacterialHemolysin ProteinsHumansInfantInfant, NewbornMaleAdhesins, BacterialDNA, BacterialeaeA protein, E coliEscherichia coli ProteinsHemolysin ProteinsHlya protein, E coliShiga ToxinVirulence Factors

Identifiers

PMID21292859
PMCPMC3133664
OpenAlexW2104690017

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.