ArticleCell regulation1990
The adhesion plaque protein, talin, is phosphorylated in vivo in chicken embryo fibroblasts exposed to a tumor-promoting phorbol ester.
Article in Cell regulation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Mechanisms of talin-dependent integrin signaling and crosstalk.Biochimica et biophysica acta · 2014Review
- Significance of talin in cancer progression and metastasis.International review of cell and molecular biology · 2011Review
- Cell-matrix adhesions differentially regulate fascin phosphorylation.Molecular biology of the cell · 1999Article
- Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles.The Journal of cell biology · 1998Article
- Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.Molecular biology of the cell · 1996Article
- Intermediate filament proteins in TPA-treated skeletal muscle cells in culture.Journal of muscle research and cell motility · 1996Article
- Focal adhesion as a signal transduction organelle.Cancer metastasis reviews · 1994Review
- Regulation of E-cadherin-mediated adhesion by muscarinic acetylcholine receptors in small cell lung carcinoma.The Journal of cell biology · 1993Article
- Actin-membrane coupling: a role for talin.Journal of muscle research and cell motility · 1992Article
- Changes in the cytoskeleton of 3T3 fibroblasts induced by the phosphatase inhibitor, calyculin-A.Journal of muscle research and cell motility · 1992Article
- Calcium channel blocker treatment of tumor cells induces alterations in the cytoskeleton, mobility of the integrin alpha IIb beta 3 and tumor-cell-induced platelet aggregation.Journal of cancer research and clinical oncology · 1992Article
- Accumulation of talin in nodes at the edge of the lamellipodium and separate incorporation into adhesion plaques at focal contacts in fibroblasts.The Journal of cell biology · 1991Article
- Interleukin 1 beta induces rapid phosphorylation and redistribution of talin: a possible mechanism for modulation of fibroblast focal adhesion.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Paxillin: a new vinculin-binding protein present in focal adhesions.The Journal of cell biology · 1990Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
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Abstract
Talin is a high molecular weight phosphoprotein that is localized at adhesion plaques. We have found that talin phosphorylation increases 3.0-fold upon exposure of chicken embryo fibroblasts to the tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate. Talin isolated from tumor promoter-treated cells is phosphorylated on serine and threonine residues. Vinculin, a 130 kDa talin-binding protein, also exhibits increased phosphorylation in vivo in response to tumor promoter, but to a lesser degree than does talin. Because tumor-promoting phorbol esters augment protein kinase C activity, we have compared the ability of purified protein kinase C to phosphorylate talin and vinculin in vitro. Both talin and vinculin were found to be substrates for protein kinase C; however, talin was phosphorylated to a greater extent than was vinculin. Cleavage of protein kinase C-phosphorylated talin by the calcium-dependent protease (Type II) revealed that while both the resulting 190-200 and 46 kDa proteolytic peptides were phosphorylated, the majority of label was contained within the 46-kDa fragment. Although incubation of chicken embryo fibroblasts with tumor-promoting phorbol ester induces a dramatic increase in talin phosphorylation, we detected no change in the organization of stress fibers and focal contacts in these cells. Exposure of the cells to tumor promoter did, however, result in a loss of actin and talin-rich cell surface elaborations that resemble focal contact precursor structures.
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