ArticleMolecular cell2011
Viral-mediated noisy gene expression reveals biphasic E2f1 response to MYC.
Article in Molecular cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
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- Temporal order and precision of complex stress responses in individual bacteria.Molecular systems biology · 2019Article
- Controlling Depth of Cellular Quiescence by an Rb-E2F Network Switch.Cell reports · 2017Article
- Expression level is a key determinant of E2F1-mediated cell fate.Cell death and differentiation · 2017Article
- Kinetic Modeling of ABCG2 Transporter Heterogeneity: A Quantitative, Single-Cell Analysis of the Side Population Assay.PLoS computational biology · 2016Article
- Cellular Interrogation: Exploiting Cell-to-Cell Variability to Discriminate Regulatory Mechanisms in Oscillatory Signalling.PLoS computational biology · 2016Article
- Addressing biological uncertainties in engineering gene circuits.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Stochastic sensitivity analysis and kernel inference via distributional data.Biophysical journal · 2014Article
- Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.Nature communications · 2014Article
- E2F1 is a novel fibrogenic gene that regulates cholestatic liver fibrosis through the Egr-1/SHP/EID1 network.Hepatology (Baltimore, Md.) · 2014Article
- Phenotypic signatures arising from unbalanced bacterial growth.PLoS computational biology · 2014Article
- Systematic reverse engineering of network topologies: a case study of resettable bistable cellular responses.PloS one · 2014Article
- Linear population allocation by bistable switches in response to transient stimulation.PloS one · 2014Article
- A synthetic biology approach to understanding cellular information processing.ACS synthetic biology · 2012Review
- Dynamics of protein noise can distinguish between alternate sources of gene-expression variability.Molecular systems biology · 2012Article
- Cooperative binding of transcription factors promotes bimodal gene expression response.PloS one · 2012Article
- Tension and robustness in multitasking cellular networks.PLoS computational biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Gene expression mediated by viral vectors is subject to cell-to-cell variability, which limits the accuracy of gene delivery. When coupled with single-cell measurements, however, such variability provides an efficient means to quantify signaling dynamics in mammalian cells. Here, we illustrate the utility of this approach by mapping the E2f1 response to MYC, serum stimulation, or both. Our results revealed an underappreciated mode of gene regulation: E2f1 expression first increased, then decreased as MYC input increased. This biphasic pattern was also reflected in other nodes of the network, including the miR-17-92 microRNA cluster and p19Arf. A mathematical model of the network successfully predicted modulation of the biphasic E2F response by serum and a CDK inhibitor. In addition to demonstrating how noise can be exploited to probe signaling dynamics, our results reveal how coordination of the MYC/RB/E2F pathway enables dynamic discrimination of aberrant and normal levels of growth stimulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.