ArticleNeuroscience research2011
Parallel high throughput neuronal toxicity assays demonstrate uncoupling between loss of mitochondrial membrane potential and neuronal damage in a model of HIV-induced neurodegeneration.
Article in Neuroscience research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Stress granules at the crossroads of retroviral replication and antiviral immunity: mechanisms and therapeutic opportunities.Molecular biology reports · 2026Review
- Dolutegravir induces endoplasmic reticulum stress at the blood-brain barrier.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Increased Purinergic Signaling in Human Dental Pulps With Inflammatory Pain is Sex-Dependent.The journal of pain · 2024Article
- Improved neurocognitive performance in FIV infected cats following treatment with the p75 neurotrophin receptor ligand LM11A-31.Journal of neurovirology · 2021Article
- Gracilin-Derivatives as Lead Compounds for Anti-inflammatory Effects.Cellular and molecular neurobiology · 2020Article
- Novel Positive Allosteric Modulators of Glutamate Transport Have Neuroprotective Properties in an in Vitro Excitotoxic Model.ACS chemical neuroscience · 2019Article
- Tinospora cordifolia as a potential neuroregenerative candidate against glutamate induced excitotoxicity: an in vitro perspective.BMC complementary and alternative medicine · 2018Article
- Differential regulation of macrophage phenotype by mature and pro-nerve growth factor.Journal of neuroimmunology · 2015Article
- E2F1 in neurons is cleaved by calpain in an NMDA receptor-dependent manner in a model of HIV-induced neurotoxicity.Journal of neurochemistry · 2015Article
- Antiretroviral drugs induce oxidative stress and neuronal damage in the central nervous system.Journal of neurovirology · 2014Article
- Spongionella secondary metabolites protect mitochondrial function in cortical neurons against oxidative stress.Marine drugs · 2014Article
- Mitigation of ROS insults by Streptomyces secondary metabolites in primary cortical neurons.ACS chemical neuroscience · 2014Article
- Calpain-mediated degradation of MDMx/MDM4 contributes to HIV-induced neuronal damage.Molecular and cellular neurosciences · 2013Article
- Article
- Differential roles for caspase-mediated and calpain-mediated cell death in 1- and 3-week-old rat cortical cultures.Neuroreport · 2012Article
- Independent effects of HIV, aging, and HAART on brain volumetric measures.Journal of acquired immune deficiency syndromes (1999) · 2012Article
- Activation status of integrated stress response pathways in neurones and astrocytes of HIV-associated neurocognitive disorders (HAND) cortex.Neuropathology and applied neurobiology · 2012Article
- Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- NMDA receptor modulation by the neuropeptide apelin: implications for excitotoxic injury.Journal of neurochemistry · 2011Article
- Site-specific hyperphosphorylation of pRb in HIV-induced neurotoxicity.Molecular and cellular neurosciences · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Neurocognitive deficits seen in HIV-associated neurocognitive disorders (HANDs) are attributed to the release of soluble factors from CNS-resident, HIV-infected and/or activated macrophages and microglia. To study HIV-associated neurotoxicity, we used our in vitro model in which primary rat neuronal/glial cultures are treated with supernatants from cultured human monocyte-derived macrophages, infected with a CNS-isolated HIV-1 strain (HIV-MDM). We found that neuronal damage, detected as a loss of microtubule-associated protein-2 (MAP2), begins as early as 2h and is preceded by a loss of mitochondrial membrane potential (Δψ(m)). Interestingly, inhibitors of calpains, but not inhibitors of caspases, blocked MAP2 loss, however neither type of inhibitor prevented the loss of Δψ(m). To facilitate throughput for these studies, we refined a MAP2 cell-based-ELISA whose data closely compare with our standardized method of hand counting neurons. In addition, we developed a tetramethyl rhodamine methyl ester (TMRM)-based multi-well fluorescent plate assay for the evaluation of whole culture Δψ(m). Together, these findings indicate that calpain activation and loss of Δψ(m) may be parallel pathways to death in HIV-MDM-treated neurons and also demonstrate the validity of plate assays for assessing multiple experimental parameters as is useful for screening neurotherapeutics for neuronal damage and death.
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