Evidence map›Paper›PMID 21291593›Full record

ArticleEmerging infectious diseases2011

Next-generation sequencing of Coccidioides immitis isolated during cluster investigation.

David M Engelthaler, Tom Chiller, James A Schupp, Joshua Colvin, Stephen M Beckstrom-Sternberg, Elizabeth M Driebe, Tracy Moses, Waibhav Tembe, Shripad Sinari, James S Beckstrom-Sternberg and 7 more

Abstract readEvaluation Study
In one paragraph

Article in Emerging infectious diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Genetic Characterization ofJournal of fungi (Basel, Switzerland) · 2022
    Article
  5. Review
  6. Article
  7. Review
  8. Coccidioides ecology and genomics.Medical mycology · 2019
    Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Valley fever: finding new places for an old disease: Coccidioides immitis found in Washington State soil associated with recent human infection.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

David M EngelthalerTranslational Genomics Research Institute, Phoenix, Arizona, USA.
Tom Chiller
James A Schupp
Joshua Colvin
Stephen M Beckstrom-Sternberg
Elizabeth M Driebe
Tracy Moses
Waibhav Tembe
Shripad Sinari
James S Beckstrom-Sternberg
Alexis Christoforides
John V Pearson
John Carpten
Paul Keim
Ashley Peterson
Dawn Terashita
S Arunmozhi Balajee

Funding

Washington University Institute of Clinical and Translational SciencesUL1TR000448 · NCATS · WASHINGTON UNIVERSITY · PI EVANOFF, BRADLEY A · 2012 to 2016
$41.4M
Highly Parallel Supercomputing for Translational ResearchS10RR025056 · NCRR · TRANSLATIONAL GENOMICS RESEARCH INST · PI SUH, EDWARD B · 2008 to 2008
$2.0M
64-Bit High Performance ComputingS10RR023390 · NCRR · TRANSLATIONAL GENOMICS RESEARCH INST · PI SUH, EDWARD B · 2007 to 2007
$403k
NCATS NIH HHS UL1 TR000448NCRR NIH HHS 1S10RR023390-01NCRR NIH HHS 1S10RR25056-01NCRR NIH HHS S10 RR023390NCRR NIH HHS S10 RR025056
6 · The paper itself

Abstract

Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.

Indexed as

Cluster AnalysisCoccidioidesCoccidioidomycosisDNA, FungalGenome, FungalGenotypeHumansMolecular EpidemiologyMycological Typing TechniquesOrgan TransplantationPhylogenyPolymorphism, Single NucleotideSequence AlignmentSequence Analysis, DNASpecies SpecificityDNA, Fungal

Identifiers

PMID21291593
PMCPMC3204756

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.