ArticleJournal of proteome research2011
Definition of the extracellular proteome of pathogenic-phase Histoplasma capsulatum.
Article in Journal of proteome research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Common virulence factors betweenCurrent research in microbial sciences · 2024Review
- Fungal mechanisms of intracellular survival: what can we learn from bacterial pathogens?Infection and immunity · 2023Review
- Pathogenicity & virulence ofVirulence · 2022Review
- A roadmap to understanding diversity and function of coral reef-associated fungi.FEMS microbiology reviews · 2022Article
- Cbp1, a fungal virulence factor under positive selection, forms an effector complex that drives macrophage lysis.PLoS pathogens · 2022Article
- An Indian lineage of Histoplasma with strong signatures of differentiation and selection.Fungal genetics and biology : FG & B · 2022Article
- Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Diagnosis of histoplasmosis: current status and perspectives.Applied microbiology and biotechnology · 2021Review
- Sensing the heat and the host: Virulence determinants ofVirulence · 2019Review
- Molecular regulation of Histoplasma dimorphism.Current opinion in microbiology · 2019Review
- How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.Journal of molecular biology · 2019Review
- Flying under the radar: Histoplasma capsulatum avoidance of innate immune recognition.Seminars in cell & developmental biology · 2019Review
- O-Mannosylation of Proteins EnablesmBio · 2018Article
- Differentiation of the fungus Histoplasma capsulatum into a pathogen of phagocytes.Current opinion in microbiology · 2017Review
- Eng1 and Exg8 Are the Major β-Glucanases Secreted by the Fungal PathogenThe Journal of biological chemistry · 2017Article
- Regulation of the fungal secretome.Current genetics · 2016Review
- Article
- Article
- Revisiting old friends: Developments in understanding Histoplasma capsulatum pathogenesis.Journal of microbiology (Seoul, Korea) · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The dimorphic fungal pathogen Histoplasma capsulatum causes respiratory and systemic disease. Within the mammalian host, pathogenic Histoplasma yeast infect, replicate within, and ultimately kill host phagocytes. Surprisingly, few factors have been identified that contribute to Histoplasma virulence. To address this deficiency, we have defined the constituents of the extracellular proteome using LC-MS/MS analysis of the proteins in pathogenic-phase culture filtrates of Histoplasma. In addition to secreted Cbp1, the extracellular proteome of pathogenic Histoplasma yeast consists of 33 deduced proteins. The proteins include glycanases, extracellular enzymes related to oxidative stress defense, dehydrogenase enzymes, chaperone-like factors, and five novel culture filtrate proteins (Cfp's). For independent verification of proteomics-derived identities, we employed RNA interference (RNAi)-based depletion of candidate factors and showed loss of specific proteins from the cell-free culture filtrate. Quantitative RT-PCR revealed the expression of 10 of the extracellular factors was particularly enriched in pathogenic yeast cells as compared to nonpathogenic Histoplasma mycelia, suggesting that these proteins are linked to Histoplasma pathogenesis. In addition, Histoplasma yeast express these factors within macrophages and during infection of murine lungs. As extracellular proteins are positioned at the interface between host and pathogen, the definition of the pathogenic-phase extracellular proteome provides a foundation for the molecular dissection of how Histoplasma alters the host-pathogen interaction to its advantage.
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Registered trials
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