Evidence map›Paper›PMID 21290472›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010

Improved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophils.

Andrea J Clark, Roberto Romero, Howard R Petty

Abstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Andrea J ClarkDepartment of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA.
Roberto Romero
Howard R Petty

Funding

Signaling Dynamics of Leukocyte-Tumor Cell InteractionsR01CA074120 · NCI · WAYNE STATE UNIVERSITY · PI PETTY, HOWARD R · 1998 to 2008
$2.1M
Intramural NIH HHSNCI NIH HHS R01 CA074120NICHD NIH HHS N01-HD-2-3342PHS HHS WSU04055
6 · The paper itself

Abstract

Kinetic studies of nicotinamide adenine dinucleotide phosphate autofluorescence have been conducted in adherent neutrophils using an improved microscopic photometry system incorporating low noise excitation and detection systems. Dynamic autofluorescence oscillations were found with periods ranging from ∼4 min to ∼10 s. The largest portion of the population of oscillating neutrophils (32%) had periods near 2 min. The next largest group at 25% exhibited periods of 1 min or less. These oscillations could not be accounted for by instrument artifacts, cell shape changes away from the focal plane, or other factors. They disappeared when detergent was added to oscillating cells. Higher-frequency oscillations disappeared as cells changed shape, indicating a correlation between these two processes. This approach provides a reliable method to monitor this cellular property.

Indexed as

FemaleFluorometryHumansKineticsMaleNADNADPNeutrophilsNADNADP

Identifiers

PMID21290472
PMCPMC3080018

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.