ArticleJournal of immunology (Baltimore, Md. : 1950)2011
Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells.
Article in Journal of immunology (Baltimore, Md. : 1950), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Knockdown of TMEM30A in renal tubular epithelial cells leads to reduced glucose absorption.BMC nephrology · 2023Article
- Physiological and Pathological Functions of TMEM30A: An Essential Subunit of P4-ATPase Phospholipid Flippases.Journal of lipids · 2023Review
- Dooming Phagocyte Responses: Inflammatory Effects of Endogenous Oxidized Phospholipids.Frontiers in endocrinology · 2021Review
- Interpretable Machine Learning Reveals Dissimilarities Between Subtypes of Autism Spectrum Disorder.Frontiers in genetics · 2021Article
- Identification of biomarkers associated with extracellular vesicles based on an integrative pan-cancer bioinformatics analysis.Medical oncology (Northwood, London, England) · 2020Article
- Disruption of Tmem30a results in cerebellar ataxia and degeneration of Purkinje cells.Cell death & disease · 2018Article
- The effect of oxidized phospholipids on phenotypic polarization and function of macrophages.Free radical biology & medicine · 2017Review
- Preferential hydrolysis of truncated oxidized glycerophospholipids by lysosomal phospholipase A2.Journal of lipid research · 2017Article
- Context-Dependent Role of Oxidized Lipids and Lipoproteins in Inflammation.Trends in endocrinology and metabolism: TEM · 2017Review
- Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping.Progress in lipid research · 2016Review
- Tumor-selective anti-cancer effects of the synthetic alkyl phosphocholine analog CLR1404 in neuroblastoma.American journal of cancer research · 2015Article
- Lipid peroxidation generates biologically active phospholipids including oxidatively N-modified phospholipids.Chemistry and physics of lipids · 2014Review
- P4-ATPases: lipid flippases in cell membranes.Pflugers Archiv : European journal of physiology · 2014Review
- Lipids and cell death in yeast.FEMS yeast research · 2014Review
- Ion channels and osteoarthritic pain: potential for novel analgesics.Current pain and headache reports · 2013Review
- Alteration of plasma membrane organization by an anticancer lysophosphatidylcholine analogue induces intracellular acidification and internalization of plasma membrane transporters in yeast.The Journal of biological chemistry · 2013Article
- Circulating biologically active oxidized phospholipids show on-going and increased oxidative stress in older male mice.Redox biology · 2013Article
- Bioactive oxidatively truncated phospholipids in inflammation and apoptosis: formation, targets, and inactivation.Biochimica et biophysica acta · 2012Review
- Structural identification and cardiovascular activities of oxidized phospholipids.Circulation research · 2012Review
- Role of phospholipid oxidation products in atherosclerosis.Circulation research · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. We show that import of choline phospholipids into S. cerevisiae ΔLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous. TMEM30a-GFP chimeras expressed in mammalian cells localized in plasma membranes, as well as internal organelles, and ectopic TMEM30a expression promoted uptake of exogenous choline and ethanolamine phospholipids. Short hairpin RNA knockdown of TMEM30a reduced fluorescent choline phospholipid and [(3)H]PAF import. This knockdown also reduced mitochondrial depolarization from exogenous Edelfosine or the mitotoxic oxidatively truncated phospholipid azelaoyl phosphatidylcholine, and the knockdown reduced apoptosis in response to these two phospholipids. These results show that extracellular choline phospholipids with short sn-2 residues can have intracellular roles and sites of metabolism because they are transport substrates for a TMEM30a phospholipid import system. Variation in this mechanism could limit sensitivity to short chain choline phospholipids such as Edelfosine, PAF, and proapoptotic phospholipids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.