ArticleJournal of clinical microbiology2011
Discovery and phylogenetic analysis of novel members of class b enterotoxigenic Escherichia coli adhesive fimbriae.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Emerging and Underdetected Colonization Factors in EnterotoxigenicMicroorganisms · 2026Review
- Genomics and pathotypes of the many faces of Escherichia coli.FEMS microbiology reviews · 2022Article
- Long-read-sequenced reference genomes of the seven major lineages of enterotoxigenic Escherichia coli (ETEC) circulating in modern time.Scientific reports · 2021Article
- Complete Whole Genome Sequences ofMicroorganisms · 2021Article
- Genomic Epidemiology and Antimicrobial Susceptibility Profile of EnterotoxigenicFrontiers in microbiology · 2021Article
- Multidrug-Resistant Lineage of EnterotoxigenicFrontiers in microbiology · 2021Article
- Colonization factor CS30 from enterotoxigenicVirulence · 2020Article
- Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile.PLoS neglected tropical diseases · 2019Article
- The bile salt glycocholate induces global changes in gene and protein expression and activates virulence in enterotoxigenic Escherichia coli.Scientific reports · 2019Article
- Coli Surface Antigen 26 Acts as an Adherence Determinant of EnterotoxigenicFrontiers in microbiology · 2018Article
- Identification and characterization of the novel colonization factor CS30 based on whole genome sequencing in enterotoxigenic Escherichia coli (ETEC).Scientific reports · 2017Article
- Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology.Scientific reports · 2017Article
- Murine immunization with CS21 pili or LngA major subunit of enterotoxigenic Escherichia coli (ETEC) elicits systemic and mucosal immune responses and inhibits ETEC gut colonization.Veterinary microbiology · 2017Article
- Antibodies Damage the Resilience of Fimbriae, Causing Them To Be Stiff and Tangled.Journal of bacteriology · 2017Article
- Diarrheagenic Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2016Review
- Chaperone-Usher Pili Loci of Colonization Factor-Negative Human EnterotoxigenicFrontiers in cellular and infection microbiology · 2016Article
- A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.Pathogens and disease · 2015Article
- Antibody-mediated disruption of the mechanics of CS20 fimbriae of enterotoxigenic Escherichia coli.Scientific reports · 2015Article
- A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
- Structure and function of enterotoxigenic Escherichia coli fimbriae from differing assembly pathways.Molecular microbiology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterotoxigenic Escherichia coli (ETEC) is recognized to be a common cause of acute watery diarrhea in children from developing countries. Colonization factors (CFAs) have been identified predominantly in ETEC isolates secreting heat-stable enterotoxin (ST) or cosecreting ST with a heat-labile toxin (LT). We hypothesized that LT-only-secreting ETEC produces unique colonization factors not previously described in ST and LTST-secreting ETEC. A set of degenerate primers based on nucleotide sequence similarities between the major structural genes of CS20 (csnA), CS18 (fotA), CS12 (cswA), and porcine antigen 987 (fasA) was developed and used to screen a collection of 266 LT-secreting ETEC isolates in which no known CFA was detected. PCR-amplified products of different molecular masses were obtained from 49 (18.4%) isolates. Nucleotide sequence analysis of the PCR amplicons followed by GenBank nucleotide BLASTn analysis revealed five novel DNA sequences; translated amino acid BLASTx analysis confirmed sequence similarity to class 1b major structural proteins encoded by csnA, fotA, and fasA. Strains expressing the novel CFAs were phylotyped and analyzed using multilocus sequence typing (MLST; Achtman scheme), and the types detected were compared to those of a collection of archived global E. coli strains. In conclusion, application of the degenerate primer sets to ETEC isolates from surveillance studies increased the total number of ETEC isolates with detectable CFAs by almost 20%. Additionally, MLST analysis suggests that for many CFAs, there may be a requirement for certain genetic backgrounds to acquire and maintain plasmids carrying genes encoding CFAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.