ArticleMolecular biology of the cell2011
Dual function of Rpn5 in two PCI complexes, the 26S proteasome and COP9 signalosome.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- The multifunctional role of the COP9 signalosome in eukaryotic development, immunity, and environmental adaptation.Planta · 2025Review
- Csn5 Depletion Reverses Mitochondrial Defects in GCN5-NullInternational journal of molecular sciences · 2025Article
- Redox-driven regulation of UCHL3/Yuh1 influences mitochondrial health via the NEDD8/Rub1 pathway.Redox biology · 2025Article
- The Ubiquitin-like Proteins ofBiomolecules · 2023Review
- Strategies for Monitoring "Ubiquitin C-Terminal Hydrolase 1" (Yuh1) Activity.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Cellular and Developmental Roles of Cullins, Neddylation, and the COP9 Signalosome inFrontiers in physiology · 2022Review
- Article
- An evolutionarily distinct chaperone promotes 20S proteasome α-ring assembly in plants.Journal of cell science · 2020Article
- The necessity of NEDD8/Rub1 for vitality and its association with mitochondria-derived oxidative stress.Redox biology · 2020Review
- IKK-Mediated Regulation of the COP9 Signalosome via Phosphorylation of CSN5.Journal of proteome research · 2020Article
- Article
- Improved discovery of genetic interactions using CRISPRiSeq across multiple environments.Genome research · 2019Article
- Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status.Redox biology · 2019Article
- Proteasome Structure and Assembly.Journal of molecular biology · 2017Review
- De Novo Disruption of the Proteasome Regulatory Subunit PSMD12 Causes a Syndromic Neurodevelopmental Disorder.American journal of human genetics · 2017Article
- The devil is in the details: comparison between COP9 signalosome (CSN) and the LID of the 26S proteasome.Current genetics · 2016Review
- The protein quality control machinery regulates its misassembled proteasome subunits.PLoS genetics · 2015Article
- Base-CP proteasome can serve as a platform for stepwise lid formation.Bioscience reports · 2015Article
- The NEDD8 modification pathway in plants.Frontiers in plant science · 2014Review
- Article
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subunit composition and architectural structure of the 26S proteasome lid is strictly conserved between all eukaryotes. This eight-subunit complex bears high similarity to the eukaryotic translation initiation factor 3 and to the COP9 signalosome (CSN), which together define the proteasome CSN/COP9/initiation factor (PCI) troika. In some unicellular eukaryotes, the latter two complexes lack key subunits, encouraging questions about the conservation of their structural design. Here we demonstrate that, in Saccharomyces cerevisiae, Rpn5 plays dual roles by stabilizing proteasome and CSN structures independently. Proteasome and CSN complexes are easily dissected, with Rpn5 the only subunit in common. Together with Rpn5, we identified a total of six bona fide subunits at roughly stoichiometric ratios in isolated, affinity-purified CSN. Moreover, the copy of Rpn5 associated with the CSN is required for enzymatic hydrolysis of Rub1/Nedd8 conjugated to cullins. We propose that multitasking by a single subunit, Rpn5 in this case, allows it to function in different complexes simultaneously. These observations demonstrate that functional substitution of subunits by paralogues is feasible, implying that the canonical composition of the three PCI complexes in S. cerevisiae is more robust than hitherto appreciated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.