ArticleMethods (San Diego, Calif.)2011
Bi-allelic gene targeting in mouse embryonic stem cells.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Fine-Tuning Homology-Directed Repair (HDR) for Precision Genome Editing: Current Strategies and Future Directions.International journal of molecular sciences · 2025Review
- Article
- Strategies for precise gene edits in mammalian cells.Molecular therapy. Nucleic acids · 2023Review
- Lack of evidence for participation of TMEM150C in sensory mechanotransduction.The Journal of general physiology · 2022Article
- A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damage.Nature cancer · 2021Article
- The Rab32/BLOC-3-dependent pathway mediates host defense against different pathogens in human macrophages.Science advances · 2021Article
- Loss of IL-10 signaling in macrophages limits bacterial killing driven by prostaglandin E2.The Journal of experimental medicine · 2020Article
- Use of two gRNAs for CRISPR/Cas9 improves bi-allelic homologous recombination efficiency in mouse embryonic stem cells.Genesis (New York, N.Y. : 2000) · 2018Article
- An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.Nucleic acids research · 2017Article
- Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis.Nature communications · 2017Article
- One-step generation of conditional and reversible gene knockouts.Nature methods · 2017Article
- Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.Scientific reports · 2015Article
- Permissive and restricted virus infection of murine embryonic stem cells.The Journal of general virology · 2012Article
- Utility of genetically modified mice for understanding the neurobiology of substance use disorders.Human genetics · 2012Review
- Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.Nucleic acids research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The EUCOMM and KOMP programs have generated targeted conditional alleles in mouse embryonic stem cells for nearly 10,000 genes. The availability of these stem cell resources will greatly accelerate the functional analysis of genes in mice and in cultured cells. We present a method for conditional ablation of genes in ES cells using vectors and targeted clones from the EUCOMM and KOMP conditional resources. Inducible homozygous cells described here provide a precisely controlled experimental system to study gene function in a model cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.