Evidence map›Paper›PMID 21287326›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2011

In vitro Treg suppression assays.

Lauren W Collison, Dario A A Vignali

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.

0numbers the graph read from it
0cells of the map it votes in
127citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

127 citing papers in PubMed.

  1. Article
  2. Obesity Promotes Lung Carcinogenesis Through Airway Immune Dysfunction.Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Local control of T cell fate in lymph nodes safely and durably reverses myelin-driven autoimmunity.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Generation of Human Chimeric Antigen Receptor Regulatory T Cells.Journal of visualized experiments : JoVE · 2025
    Article
  19. Review
  20. Article

67 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lauren W CollisonDepartment of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Dario A A Vignali

Funding

Viral Vector Technology (VVTSR)P30CA021765 · NCI · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI SUZANNE J. BAKER · 1985 to 2026
$166.9M
Molecular Control of TCR Signaling and FunctionR01AI052199 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI VIGNALI, DARIO AA · 2003 to 2015
$3.8M
Multimolecular Interactions During TCR RecognitionR01AI039480 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI VIGNALI, DARIO AA · 2001 to 2011
$3.6M
A Regulatory Triad: IL35, Tregs and iTr35R01AI091977 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI VIGNALI, DARIO AA · 2011 to 2015
$2.0M
Interleukin-35 and the tumor microenvironmentR01CA203689 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI VIGNALI, DARIO AA · 2016 to 2020
$1.9M
Molecular Control of TCR Signaling and FunctionR56AI052199 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI VIGNALI, DARIO AA · 2010 to 2010
$435k
Control of CD4+ T cell Islet Entry and DiabetogenicityR56AI072239 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI VIGNALI, DARIO AA · 2008 to 2008
$418k
Molecular Control of TCR Signaling and FunctionR21AI052199 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI VIGNALI, DARIO AA · 2002 to 2002
$375k
Pairing of EBI-3 and IL12 p35 To Yield A Novel Regulatory T cell CytokineF32AI072816 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI COLLISON, LAUREN W · 2007 to 2009
$142k
MULTIMOLECULAR INTERACTIONS DURING TCR RECOGNITIONR29AI039480 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI VIGNALI, DARIO AA · 1996 to 2000
$108k
NCI NIH HHS CA21765NCI NIH HHS P30 CA021765NCI NIH HHS R01 CA203689NIAID NIH HHS AI072239NIAID NIH HHS AI39480NIAID NIH HHS AI52199NIAID NIH HHS F32 AI072816NIAID NIH HHS R01 AI039480NIAID NIH HHS R01 AI052199NIAID NIH HHS R01 AI091977NIAID NIH HHS R21 AI052199NIAID NIH HHS R29 AI039480NIAID NIH HHS R56 AI052199NIAID NIH HHS R56 AI072239
6 · The paper itself

Abstract

Determining the activity of a regulatory T-cell population in vitro is often the first step in analyzing its function. To obtain reliable and reproducible results, it is critical to follow the protocol that is most applicable to your experimental question. We have outlined below a basic in vitro suppression assay as well as a variety of alternative/additional protocols that can be utilized alone or in combination as desired.

Indexed as

Cytological TechniquesAnimalsAntigen PresentationLymphocyte ActivationT-Lymphocytes, Regulatory

Identifiers

PMID21287326
PMCPMC3043080

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.