ArticleApplied microbiology and biotechnology2011
Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy.
Article in Applied microbiology and biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Expanding the substrate spectrum in engineered Pseudomonas taiwanensis for efficient production of 4-coumarate from lignocellulosic sugars.Microbial cell factories · 2026Article
- Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida.Journal of biological engineering · 2025Article
- Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems.Chembiochem : a European journal of chemical biology · 2022Article
- Advances in engineering microbial biosynthesis of aromatic compounds and related compounds.Bioresources and bioprocessing · 2021Review
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Review
- High-Yield Production of 4-Hydroxybenzoate From Glucose or Glycerol by an EngineeredFrontiers in bioengineering and biotechnology · 2019Article
- Production of 4-Hydroxybenzoic Acid by an Aerobic Growth-Arrested Bioprocess Using Metabolically Engineered Corynebacterium glutamicum.Applied and environmental microbiology · 2018Article
- Genomic Analysis ofFrontiers in microbiology · 2018Article
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Engineered Microorganisms for the Production of Food Additives Approved by the European Union-A Systematic Analysis.Frontiers in microbiology · 2018Review
- Efficient, environmentally-friendly and specific valorization of lignin: promising role of non-radical lignolytic enzymes.World journal of microbiology & biotechnology · 2017Review
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2016Article
- Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system.Biotechnology for biofuels · 2016Article
- Pseudomonas putida-a versatile host for the production of natural products.Applied microbiology and biotechnology · 2015Review
- Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions.BMC biotechnology · 2014Article
- De novo production of the monoterpenoid geranic acid by metabolically engineered Pseudomonas putida.Microbial cell factories · 2014Article
- Production of aromatic compounds by metabolically engineered Escherichia coli with an expanded shikimate pathway.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The key precursors for p-hydroxybenzoate production by engineered Pseudomonas putida S12 are phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P), for which the pentose phosphate (PP) pathway is an important source. Since PP pathway fluxes are typically low in pseudomonads, E4P and PEP availability is a likely bottleneck for aromatics production which may be alleviated by stimulating PP pathway fluxes via co-feeding of pentoses in addition to glucose or glycerol. As P. putida S12 lacks the natural ability to utilize xylose, the xylose isomerase pathway from E. coli was introduced into the p-hydroxybenzoate producing strain P. putida S12palB2. The initially inefficient xylose utilization was improved by evolutionary selection after which the p-hydroxybenzoate production was evaluated. Even without xylose-co-feeding, p-hydroxybenzoate production was improved in the evolved xylose-utilizing strain, which may indicate an intrinsically elevated PP pathway activity. Xylose co-feeding further improved the p-hydroxybenzoate yield when co-fed with either glucose or glycerol, up to 16.3 Cmol% (0.1 g p-hydroxybenzoate/g substrate). The yield improvements were most pronounced with glycerol, which probably related to the availability of the PEP precursor glyceraldehyde-3-phosphate (GAP). Thus, it was demonstrated that the production of aromatics such as p-hydroxybenzoate can be improved by co-feeding different carbon sources via different and partially artificial pathways. Moreover, this approach opens new perspectives for the efficient production of (fine) chemicals from renewable feedstocks such as lignocellulose that typically has a high content of both glucose and xylose and (crude) glycerol.
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