ArticleMarine biotechnology (New York, N.Y.)2011
Transient gene expression system established in Porphyra yezoensis is widely applicable in Bangiophycean algae.
Article in Marine biotechnology (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 47 citations in OpenAlex.
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- High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation in the model microalga Phaeodactylum tricornutum.Microbial cell factories · 2018Article
- Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis.Marine biotechnology (New York, N.Y.) · 2017Article
- Understanding nitrate assimilation and its regulation in microalgae.Frontiers in plant science · 2015Review
- A codon-optimized bacterial antibiotic gene used as selection marker for stable nuclear transformation in the marine red alga Pyropia yezoensis.Marine biotechnology (New York, N.Y.) · 2014Article
- Tipping points in seaweed genetic engineering: scaling up opportunities in the next decade.Marine drugs · 2014Review
- A technical breakthrough close at hand: feasible approaches toward establishing a gene-targeting genetic transformation system in seaweeds.Frontiers in plant science · 2014Article
- Characterization of the sporophyte-preferential gene promoter from the red alga Porphyra yezoensis using transient gene expression.Marine biotechnology (New York, N.Y.) · 2013Article
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4 authors at 2 institutions in 1 country.
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Abstract
The establishment of transient gene expression systems in the marine red macroalga Porphyra yezoensis has been useful for the molecular analysis of cellular processes in this species. However, there has been no successful report about the expression of foreign genes in other red macroalgae, which has impeded the broader understanding of the molecular biology of these species. We therefore examined whether the P. yezoensis transient gene expression system was applicable to other red macroalgae. The results indicated that a codon-optimized GUS, designated PyGUS, and plant-adapted sGFP(S65T) were successfully expressed under the control of the P. yezoensis PyAct1 promoter in gametophytic cells of six Porphyra species and also in Bangia fuscopurpurea, all of which are classified as Bangiophyceae. In contrast, there were no reporter-expressing cells in the Florideophycean algae examined. These results indicate the availability of PyGUS and sGFP as reporters and the 5' upstream region of the PyAct1 gene as a heterologous promoter for transient gene expression in Bangiophycean algae, which could provide a clue to the efficient expression of foreign genes and transformation in marine red macroalgae.
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