ArticleCardiovascular research2011
Two distinct phases of calcium signalling under flow.
Article in Cardiovascular research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Mitochondria regulate neutrophil activation by generating ATP for autocrine purinergic signaling.The Journal of biological chemistry · 2014Trial
- Platelet-derived microvesicles induce calcium oscillations and promote VSMC migrationTheranostics · 2021Article
- Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress.Biomolecules · 2019Article
- A Membrane-Bound Biosensor Visualizes Shear Stress-Induced Inhomogeneous Alteration of Cell Membrane Tension.iScience · 2018Article
- FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation.Communications biology · 2018Article
- Mitochondrial CaJournal of the Royal Society, Interface · 2017Review
- NOTCH1 is a mechanosensor in adult arteries.Nature communications · 2017Article
- Cdc42 activation couples fluid shear stress to apical endocytosis in proximal tubule cells.Physiological reports · 2017Article
- Visualizing the spatiotemporal map of Rac activation in bovine aortic endothelial cells under laminar and disturbed flows.PloS one · 2017Article
- Ion Channels in Endothelial Responses to Fluid Shear Stress.Physiology (Bethesda, Md.) · 2016Review
- Endothelial mitochondria regulate the intracellular Ca2+ response to fluid shear stress.American journal of physiology. Cell physiology · 2016Article
- Global changes of phospholipids identified by MALDI imaging mass spectrometry in a mouse model of Alzheimer's disease.Journal of lipid research · 2016Article
- Mechanosensitive TRPM7 mediates shear stress and modulates osteogenic differentiation of mesenchymal stromal cells through Osterix pathway.Scientific reports · 2015Article
- Lymphatic network in atherosclerosis: the underestimated path.Future science OA · 2015Review
- Measurement of shear stress-mediated intracellular calcium dynamics in human dermal lymphatic endothelial cells.American journal of physiology. Heart and circulatory physiology · 2015Article
- Laser-induced shockwave paired with FRET: a method to study cell signaling.Microscopy research and technique · 2015Article
- RhoA and membrane fluidity mediates the spatially polarized Src/FAK activation in response to shear stress.Scientific reports · 2014Article
- High-throughput optical screening of cellular mechanotransduction.Nature photonics · 2014Article
- The role of mechanical tension on lipid raft dependent PDGF-induced TRPC6 activation.Biomaterials · 2014Article
- Ca2+/calmodulin-dependent protein kinase kinase β phosphorylation of Sirtuin 1 in endothelium is atheroprotective.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
aimsHigh shear stress (HSS) can have significant impact on angiogenesis and atherosclerosis in collateral arteries near the bifurcation and curvature regions. Here, we investigate the spatiotemporal pattern of HSS-induced intracellular calcium alteration. METHODS AND
resultsGenetically encoded biosensors based on fluorescence resonance energy transfer were targeted in the cytoplasm and the endoplasmic reticulum (ER) to visualize the subcellular calcium dynamics in bovine aortic endothelial cells under HSS (65 dyn/cm(2)). Upon HSS application, the intracellular Ca(2+) concentration ([Ca(2+)](i)) increased immediately and maintained a sustained high level, while the ER-stored calcium had a significant decrease only after 300 s. The perturbation of calcium influx across the plasma membrane (PM) by the removal of extracellular calcium or the blockage of membrane channels inhibited the early phase of [Ca(2+)](i) increase upon HSS application, which was further shown to be sensitive to the magnitudes of shear stress and the integrity of cytoskeletal support. In contrast, Src, phospholipase C(PLC), and the inositol 1,4,5-trisphosphate receptor (IP(3)R) can regulate the late phase of HSS-induced [Ca(2+)](i) increase via the promotion of the ER calcium efflux.
conclusionThe HSS-induced [Ca(2+)](i) increase consists of two well-co-ordinated phases with different sources and mechanisms: (i) an early phase due to the calcium influx across the PM which is dependent on the mechanical impact and cytoskeletal support and (ii) a late phase originated from the ER-calcium efflux which is regulated by the Src, PLC, and IP(3)R signalling pathway. Therefore, our work presented new molecular-level insights into systematic understanding of mechanotransduction in cardiovascular systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.