ArticlePloS one2011
Protein-protein interactions within late pre-40S ribosomes.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Ribosome Biogenesis and Cancer: Insights into NOB1 and PNO1 Mechanisms.Current pharmaceutical design · 2024Review
- A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head.PLoS genetics · 2023Article
- A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head.bioRxiv : the preprint server for biology · 2023Article
- Inhibition of ribosome assembly factor PNO1 by CRISPR/Cas9 technique suppresses lung adenocarcinoma and Notch pathway: Clinical application.Journal of cellular and molecular medicine · 2023Article
- Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release.Cell reports · 2022Article
- The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation.RNA biology · 2022Article
- Quality control of 40S ribosome head assembly ensures scanning competence.The Journal of cell biology · 2020Article
- Role of the 40S beak ribosomal protein eS12 in ribosome biogenesis and function inRNA biology · 2020Article
- Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy.Molecules (Basel, Switzerland) · 2020Article
- A kinase-dependent checkpoint prevents escape of immature ribosomes into the translating pool.PLoS biology · 2019Article
- Tsr4 and Nap1, two novel members of the ribosomal protein chaperOME.Nucleic acids research · 2019Article
- Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.Nature communications · 2019Article
- An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli.Scientific reports · 2019Article
- Interactions and activities of factors involved in the late stages of human 18S rRNA maturation.RNA biology · 2019Article
- Ribosome biogenesis factor Ltv1 chaperones the assembly of the small subunit head.The Journal of cell biology · 2018Article
- Ribosomal protein uS7/Rps5 serine-223 in protein kinase-mediated phosphorylation and ribosomal small subunit maturation.Scientific reports · 2018Article
- Reference genes selection for quantitative gene expression studies in tea green leafhoppers, Empoasca onukii Matsuda.PloS one · 2018Article
- Structural and functional analysis of Utp24, an endonuclease for processing 18S ribosomal RNA.PloS one · 2018Article
- The ATPase Fap7 Tests the Ability to Carry Out Translocation-like Conformational Changes and Releases Dim1 during 40S Ribosome Maturation.Molecular cell · 2017Article
- Structural Heterogeneity in Pre-40S Ribosomes.Structure (London, England : 1993) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ribosome assembly in eukaryotic organisms requires more than 200 assembly factors to facilitate and coordinate rRNA transcription, processing, and folding with the binding of the ribosomal proteins. Many of these assembly factors bind and dissociate at defined times giving rise to discrete assembly intermediates, some of which have been partially characterized with regards to their protein and RNA composition. Here, we have analyzed the protein-protein interactions between the seven assembly factors bound to late cytoplasmic pre-40S ribosomes using recombinant proteins in binding assays. Our data show that these factors form two modules: one comprising Enp1 and the export adaptor Ltv1 near the beak structure, and the second comprising the kinase Rio2, the nuclease Nob1, and a regulatory RNA binding protein Dim2/Pno1 on the front of the head. The GTPase-like Tsr1 and the universally conserved methylase Dim1 are also peripherally connected to this second module. Additionally, in an effort to further define the locations for these essential proteins, we have analyzed the interactions between these assembly factors and six ribosomal proteins: Rps0, Rps3, Rps5, Rps14, Rps15 and Rps29. Together, these results and previous RNA-protein crosslinking data allow us to propose a model for the binding sites of these seven assembly factors. Furthermore, our data show that the essential kinase Rio2 is located at the center of the pre-ribosomal particle and interacts, directly or indirectly, with every other assembly factor, as well as three ribosomal proteins required for cytoplasmic 40S maturation. These data suggest that Rio2 could play a central role in regulating cytoplasmic maturation steps.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.