ArticlePloS one2011
Genetic deletion of laminin isoforms β2 and γ3 induces a reduction in Kir4.1 and aquaporin-4 expression and function in the retina.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Scutellarin attenuates high glucose-induced edema and inflammation in retinal Müller cells via AQP4/Kir4.1 restoration and TLR4/MyD88/NF-κB inhibition.Frontiers in pharmacology · 2026Article
- Vitreous Fibrillar Structure and Interfibrillar Composition in Adult Mice.Investigative ophthalmology & visual science · 2025Article
- Microglial depletion decreases Müller cell maturation and inner retinal vascular density.Cell communication and signaling : CCS · 2025Article
- Mural Wnt/β-catenin signaling regulates Lama2 expression to promote neurovascular unit maturation.Development (Cambridge, England) · 2022Article
- Müller Glia in Retinal Development: From Specification to Circuit Integration.Frontiers in neural circuits · 2021Review
- Laminin β2 Chain Regulates Cell Cycle Dynamics in the Developing Retina.Frontiers in cell and developmental biology · 2021Article
- Influences of advanced glycosylation end products on the inner blood-retinal barrier in a co-culture cell modelOpen life sciences · 2020Article
- Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Laminin β2 Chain Regulates Retinal Progenitor Cell Mitotic Spindle Orientation via Dystroglycan.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation.Cell death & disease · 2018Article
- Aquaporin-4 as a New Target against Methamphetamine-Induced Brain Alterations: Focus on the Neurogliovascular Unit and Motivational Behavior.Molecular neurobiology · 2018Article
- Laminin: loss-of-function studies.Cellular and molecular life sciences : CMLS · 2017Review
- Dystroglycan Suppresses Notch to Regulate Stem Cell Niche Structure and Function in the Developing Postnatal Subventricular Zone.Developmental cell · 2016Article
- The role of glial-specific Kir4.1 in normal and pathological states of the CNS.Acta neuropathologica · 2016Review
- Increased blood-brain barrier permeability and alterations in perivascular astrocytes and pericytes induced by intracisternal glutaric acid.Fluids and barriers of the CNS · 2014Article
- Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina.Development (Cambridge, England) · 2013Article
- The cell-matrix interface: a possible target for treating retinal vascular related pathologies.Journal of ophthalmic & vision research · 2012Article
- Characterization of Aquaporin 4 Protein Expression and Localization in Tissues of the Dogfish (Squalus acanthias).Frontiers in physiology · 2012Article
- Aquaporin 4 is a Ubiquitously Expressed Isoform in the Dogfish (Squalus acanthias) Shark.Frontiers in physiology · 2011Article
- Extracellular Matrix Components Regulate Cellular Polarity and Tissue Structure in the Developing and Mature Retina.Journal of ophthalmic & vision researchReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundGlial cells such as retinal Müller glial cells are involved in potassium ion and water homeostasis of the neural tissue. In these cells, inwardly rectifying potassium (Kir) channels and aquaporin-4 water channels play an important role in the process of spatial potassium buffering and water drainage. Moreover, Kir4.1 channels are involved in the maintenance of the negative Müller cell membrane potential. The subcellular distribution of Kir4.1 and aquaporin-4 channels appears to be maintained by interactions with extracellular and intracellular molecules. Laminins in the extracellular matrix, dystroglycan in the membrane, and dystrophins in the cytomatrix form a complex mediating the polarized expression of Kir4.1 and aquaporin-4 in Müller cells. METHODOLOGY/PRINCIPAL
findingsThe aim of the present study was to test the function of the β2 and γ3 containing laminins in murine Müller cells. We used knockout mice with genetic deletion of both β2 and γ3 laminin genes to assay the effects on Kir4.1 and aquaporin-4. We studied protein and mRNA expression by immunohistochemistry, Western Blot, and quantitative RT-PCR, respectively, and membrane currents of isolated cells by patch-clamp experiments. We found a down-regulation of mRNA and protein of Kir4.1 as well as of aquaporin-4 protein in laminin knockout mice. Moreover, Müller cells from laminin β2 and γ3 knockout mice had reduced Kir-mediated inward currents and their membrane potentials were more positive than those in age-matched wild-type mice.
conclusionThese findings demonstrate a strong impact of laminin β2 and γ3 subunits on the expression and function of both aquaporin-4 and Kir4.1, two important membrane proteins in Müller cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.