ArticlePloS one2011
Modulation of the major paths of carbon in photorespiratory mutants of synechocystis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 90 citations in OpenAlex.
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- A quantitative description of light-limited cyanobacterial growth using flux balance analysis.PLoS computational biology · 2024Article
- Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.Nature communications · 2024Article
- The primary carbon metabolism in cyanobacteria and its regulation.Frontiers in plant science · 2024Review
- Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, CNature plants · 2022Article
- Photoautotrophic cultivation of Chlamydomonas reinhardtii in open ponds of greenhouse.Archives of microbiology · 2021Article
- The Entner-Doudoroff Pathway Contributes to Glycogen Breakdown During High to Low COFrontiers in plant science · 2021Article
- Probing Light-Dependent Regulation of the Calvin Cycle Using a Multi-Omics Approach.Frontiers in plant science · 2021Article
- Homologs of Circadian Clock Proteins Impact the Metabolic Switch Between Light and Dark Growth in the CyanobacteriumFrontiers in plant science · 2021Article
- Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism inLife (Basel, Switzerland) · 2020Article
- Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.Metabolic engineering · 2020Article
- The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation.Nature communications · 2018Review
- TheFrontiers in plant science · 2018Article
- Systems analysis of ethanol production in the genetically engineered cyanobacteriumBiotechnology for biofuels · 2017Article
- Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis.Biotechnology for biofuels · 2017Article
- Rapid in situPlant methods · 2017Article
- Different strategies of metabolic regulation in cyanobacteria: from transcriptional to biochemical control.Scientific reports · 2016Article
- Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803.Biotechnology for biofuels · 2016Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent studies using transcript and metabolite profiles of wild-type and gene deletion mutants revealed that photorespiratory pathways are essential for the growth of Synechocystis sp. PCC 6803 under atmospheric conditions. Pool size changes of primary metabolites, such as glycine and glycolate, indicated a link to photorespiration. METHODOLOGY/PRINCIPAL
findingsThe (13)C labelling kinetics of primary metabolites were analysed in photoautotrophically grown cultures of Synechocystis sp. PCC 6803 by gas chromatography-mass spectrometry (GC-MS) to demonstrate the link with photorespiration. Cells pre-acclimated to high CO(2) (5%, HC) or limited CO(2) (0.035%, LC) conditions were pulse-labelled under very high (2% w/w) (13)C-NaHCO(3) (VHC) conditions followed by treatment with ambient (12)C at HC and LC conditions, respectively. The (13)C enrichment, relative changes in pool size, and (13)C flux of selected metabolites were evaluated. We demonstrate two major paths of CO(2) assimilation via Rubisco in Synechocystis, i.e., from 3PGA via PEP to aspartate, malate and citrate or, to a lesser extent, from 3PGA via glucose-6-phosphate to sucrose. The results reveal evidence of carbon channelling from 3PGA to the PEP pool. Furthermore, (13)C labelling of glycolate was observed under conditions thought to suppress photorespiration. Using the glycolate-accumulating ΔglcD1 mutant, we demonstrate enhanced (13)C partitioning into the glycolate pool under conditions favouring photorespiration and enhanced (13)C partitioning into the glycine pool of the glycine-accumulating ΔgcvT mutant. Under LC conditions, the photorespiratory mutants ΔglcD1 and ΔgcvT showed enhanced activity of the additional carbon-fixing PEP carboxylase pathway. CONCLUSIONS/SIGNIFICANCE: With our approach of non-steady-state (13)C labelling and analysis of metabolite pool sizes with respective (13)C enrichments, we identify the use and modulation of major pathways of carbon assimilation in Synechocystis in the presence of high and low inorganic carbon supplies.
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