ArticlePloS one2011
Glutathione transferase from Trichoderma virens enhances cadmium tolerance without enhancing its accumulation in transgenic Nicotiana tabacum.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 142 citations in OpenAlex.
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- Genome-wide identification, expression and functional analysis of glutathioneFrontiers in plant science · 2025Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- Characterization and Expression Analysis of Four Cadmium-Tolerance-Associated Genes ofBiology · 2023Article
- Transcriptomics combined with physiological analysis reveals the mechanism of cadmium uptake and tolerance inFrontiers in plant science · 2023Article
- Metal- and Organ-Specific Response to Heavy Metal-Induced Stress Mediated by Antioxidant Enzymes' Activities, Polyamines, and Plant Hormones Levels inPlants (Basel, Switzerland) · 2022Article
- A novel microRNA IamiR-4-3p from water spinach (Ipomoea aquatica Forsk.) increased Cd uptake and translocation in Arabidopsis thaliana.Environmental science and pollution research international · 2022Article
- Toxicity of Cadmium and nickel in the context of applied activated carbon biochar for improvement in soil fertility.Saudi journal of biological sciences · 2022Review
- A fungus (Frontiers in microbiology · 2022Article
- Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants.Environmental science and pollution research international · 2021Review
- Proteomics of Two Thermotolerant Isolates ofJournal of fungi (Basel, Switzerland) · 2021Article
- Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance.Antioxidants (Basel, Switzerland) · 2021Article
- Bioprospecting of Rhizosphere-Resident Fungi: Their Role and Importance in Sustainable Agriculture.Journal of fungi (Basel, Switzerland) · 2021Review
- Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots ofLife (Basel, Switzerland) · 2020Article
- Transcriptomic Analysis of TwoFrontiers in microbiology · 2020Article
- Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots ofInternational journal of molecular sciences · 2019Article
- Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco.Biology open · 2019Article
- Chelators induced uptake of cadmium and modulation of water relation, antioxidants, and photosynthetic traits of maize.Environmental science and pollution research international · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCadmium (Cd) is a major heavy metal pollutant which is highly toxic to plants and animals. Vast agricultural areas worldwide are contaminated with Cd. Plants take up Cd and through the food chain it reaches humans and causes toxicity. It is ideal to develop plants tolerant to Cd, without enhanced accumulation in the edible parts for human consumption. Glutathione transferases (GST) are a family of multifunctional enzymes known to have important roles in combating oxidative stresses induced by various heavy metals including Cd. Some GSTs are also known to function as glutathione peroxidases. Overexpression/heterologous expression of GSTs is expected to result in plants tolerant to heavy metals such as Cd.
resultsHere, we report cloning of a glutathione transferase gene from Trichoderma virens, a biocontrol fungus and introducing it into Nicotiana tabacum plants by Agrobacterium-mediated gene transfer. Transgenic nature of the plants was confirmed by Southern blot hybridization and expression by reverse transcription PCR. Transgene (TvGST) showed single gene Mendelian inheritance. When transgenic plants expressing TvGST gene were exposed to different concentrations of Cd, they were found to be more tolerant compared to wild type plants, with transgenic plants showing lower levels of lipid peroxidation. Levels of different antioxidant enzymes such as glutathione transferase, superoxide dismutase, ascorbate peroxidase, guiacol peroxidase and catalase showed enhanced levels in transgenic plants expressing TvGST compared to control plants, when exposed to Cd. Cadmium accumulation in the plant biomass in transgenic plants were similar or lower than wild-type plants.
conclusionThe results of the present study suggest that transgenic tobacco plants expressing a Trichoderma virens GST are more tolerant to Cd, without enhancing its accumulation in the plant biomass. It should be possible to extend the present results to crop plants for developing Cd tolerance and in limiting Cd availability in the food chain.
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