ArticlePloS one2011
Structure-based mutational analysis of eIF4E in relation to sbm1 resistance to pea seed-borne mosaic virus in pea.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Delineating the source of resistance to bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in common bean (Frontiers in microbiology · 2025Article
- Edited eukaryotic translation initiation factors confer resistance against maize lethal necrosis.Plant biotechnology journal · 2024Article
- eIF2Bβ confers resistance to Turnip mosaic virus by recruiting ALKBH9B to modify viral RNA methylation.Plant biotechnology journal · 2024Article
- Adaptation of turnip mosaic virus toVirus evolution · 2024Article
- Plant eIF4E isoforms as factors of susceptibility and resistance to potyviruses.Frontiers in plant science · 2023Review
- Genomics Enabled Breeding Strategies for Major Biotic Stresses in Pea (Frontiers in plant science · 2022Review
- CRISPR-Cas9 Targeting of theFrontiers in microbiology · 2022Article
- A Novel Interaction Network Used by Potyviruses in Virus-Host Interactions at the Protein Level.Viruses · 2019Article
- Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses.Plant biotechnology journal · 2019Article
- Hydrodynamic Behavior of the Intrinsically Disordered Potyvirus Protein VPg, of the Translation Initiation Factor eIF4E and of their Binary Complex.International journal of molecular sciences · 2019Article
- Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence.Plant biotechnology journal · 2019Article
- Comparative analysis of mutational robustness of the intrinsically disordered viral protein VPg and of its interactor eIF4E.PloS one · 2019Article
- A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus.Scientific reports · 2017Article
- Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation.Plant physiology · 2017Article
- Analysis of the interacting partners eIF4F and 3'-CITE required for Melon necrotic spot virus cap-independent translation.Molecular plant pathology · 2017Article
- Non-canonical Translation in Plant RNA Viruses.Frontiers in plant science · 2017Review
- Plant Translation Factors and Virus Resistance.Viruses · 2015Review
- Maize Elongin C interacts with the viral genome-linked protein, VPg, of Sugarcane mosaic virus and facilitates virus infection.The New phytologist · 2014Article
- Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.Molecular plant pathology · 2014Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPea encodes eukaryotic translation initiation factor eIF4E (eIF4E(S)), which supports the multiplication of Pea seed-borne mosaic virus (PSbMV). In common with hosts for other potyviruses, some pea lines contain a recessive allele (sbm1) encoding a mutant eIF4E (eIF4E(R)) that fails to interact functionally with the PSbMV avirulence protein, VPg, giving genetic resistance to infection. METHODOLOGY/PRINCIPAL
findingsTo study structure-function relationships between pea eIF4E and PSbMV VPg, we obtained an X-ray structure for eIF4E(S) bound to m(7)GTP. The crystallographic asymmetric unit contained eight independent copies of the protein, providing insights into the structurally conserved and flexible regions of eIF4E. To assess indirectly the importance of key residues in binding to VPg and/or m(7)GTP, an extensive range of point mutants in eIF4E was tested for their ability to complement PSbMV multiplication in resistant pea tissues and for complementation of protein translation, and hence growth, in an eIF4E-defective yeast strain conditionally dependent upon ectopic expression of eIF4E. The mutants also dissected individual contributions from polymorphisms present in eIF4E(R) and compared the impact of individual residues altered in orthologous resistance alleles from other crop species. The data showed that essential resistance determinants in eIF4E differed for different viruses although the critical region involved (possibly in VPg-binding) was conserved and partially overlapped with the m(7)GTP-binding region. This overlap resulted in coupled inhibition of virus multiplication and translation in the majority of cases, although the existence of a few mutants that uncoupled the two processes supported the view that the specific role of eIF4E in potyvirus infection may not be restricted to translation. CONCLUSIONS/SIGNIFICANCE: The work describes the most extensive structural analysis of eIF4E in relation to potyvirus resistance. In addition to defining functional domains within the eIF4E structure, we identified eIF4E alleles with the potential to convey novel virus resistance phenotypes.
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